The Effect of Cadmium Ions and Cadmium Nanoparticles on Chicken Embryos and Evaluation of Organ Accumulation

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dc.contributor.authorKenšová, Renátacs
dc.contributor.authorBlažková, Ivacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorBlažková, Luciecs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorNovotná, Mariecs
dc.contributor.authorZehnálek, Josefcs
dc.contributor.authorPohanka, Miroslavcs
dc.contributor.authorTrnková, Libušecs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue4cs
dc.coverage.volume10cs
dc.date.accessioned2021-09-03T10:52:49Z
dc.date.available2021-09-03T10:52:49Z
dc.date.issued2015-04-01cs
dc.description.abstractThe presence of metals in the organism has both positive and negative influence on the functions of the body, depending on the concentration. Cadmium is one of the metals that have irrefutable negative effect. It is highly toxic metal with long biological half-life causing damage of kidneys, bones and has carcinogenic effect. The aim of this work was the study of the effect and distribution of the cadmium in the body and its comparison with cadmium nanoparticles – CdTe quantum dots (QDs). Model organism for these experiments was chicken embryo in 16th developmental day. The distribution of cadmium was analyzed after the application of 500 uL of cadmium solution (Cd ions) in concentration 1 mg/mL or 4.5 mg/mL). This solution was applied through the small hole in the egg shell onto the chorioallantoic membrane. After 24 hours of the incubation (37.5 C, 45% rH) liver, kidney, brain and hearth were extracted. The concentration of cadmium was analyzed after the mineralization of the samples by atomic absorption spectrometry and differential pulse voltammetry. The toxicity of cadmium solution was significantly higher compared to the solution of QDs. Embryos exposed to the cadmium ions exhibited 100% mortality after 24 hours. On the contrary, the mortality of embryos exposed to QDs was 0%. The highest levels of cadmium were detected in the kidneys (125 ug per g) and in the liver (107 ug per g).en
dc.description.abstractPřítomnost kovů v organismu má pozitivní i negativní vliv na tělesné funkce, v závislosti na koncentraci. Kadmium je jeden z kovů, které mají nezvratný negativní účinek. Je to vysoce toxický kov s dlouhým biologickým poločasem a způsobuje poškození ledvin, kostí a má karcinogenní účinky. Cílem této práce bylo studium vlivu a distribuci kadmia v těle a jeho srovnání s kademnatými nanočásticemi - CdTe kvantových teček (QDS). Modelovým organismem pro tyto experimenty byly kuřecí embrya v 16. vývojovém dni. Distribuce kadmia byla analyzována po aplikaci 500 ul roztoku kadmia (Cd iontů) o koncentraci 1 mg / ml nebo 4,5 mg / ml). Tento roztok se aplikuje pomocí malého otvoru ve skořápce, na chorioallantoidní membránu. Po 24 hodinách inkubace (37,5 C, 45% RH) byly játra, ledviny, mozek a srdce extrahovány. Koncentrace kadmia, byla analyzována po mineralizaci vzorku atomovou absorpční spektrometrií a diferenční pulzní voltametrie.cs
dc.formattextcs
dc.format.extent3623-3634cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE. 2015, vol. 10, issue 4, p. 3623-3634.en
dc.identifier.issn1452-3981cs
dc.identifier.other114334cs
dc.identifier.urihttp://hdl.handle.net/11012/201453
dc.language.isoencs
dc.publisherESGcs
dc.relation.ispartofINTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCEcs
dc.relation.urihttp://www.electrochemsci.org/papers/vol10/100403623.pdfcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1452-3981/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectCadmiumen
dc.subjectQuantum Dotsen
dc.subjectChicken embryosen
dc.subjectTissueen
dc.subjectDistributionen
dc.subjectAtomic absorption spectroscopyen
dc.subjectDifferential pulse voltammetryen
dc.titleThe Effect of Cadmium Ions and Cadmium Nanoparticles on Chicken Embryos and Evaluation of Organ Accumulationen
dc.title.alternativeVliv kademnatých iontů a kademnatých nanočástic na kuřecí embrya a hodnocení akumulace v orgánechcs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-114334en
sync.item.dbtypeVAVen
sync.item.insts2021.09.03 12:52:49en
sync.item.modts2021.09.03 12:14:19en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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