Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

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dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHeger, Zbyněkcs
dc.coverage.issue40169cs
dc.coverage.volume7cs
dc.date.issued2017-01-10cs
dc.description.abstractWe describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.en
dc.formattextcs
dc.format.extent40169-1-40169-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2017, vol. 7, issue 40169, p. 40169-1-40169-13.en
dc.identifier.doi10.1038/srep40169cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.other133398cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.urihttp://hdl.handle.net/11012/69381
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://www.nature.com/articles/srep40169cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectLA-ICP-MSen
dc.subjectBEARING LIBECHOV MINIPIGen
dc.subjectMIXED-EFFECTS MODELSen
dc.subjectMETALLOTHIONEIN-OVEREXPRESSIONen
dc.subjectMELANOMA PATIENTSen
dc.subjectZINC LEVELSen
dc.subjectCOPPERen
dc.subjectMELANOBLASTOMAen
dc.subjectELEMENTSen
dc.subjectSTRESSen
dc.titleSpatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registrationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-133398en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:22en
sync.item.modts2025.01.17 15:37:00en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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