Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

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dc.contributor.authorMuchová, Johanacs
dc.contributor.authorHearnden, Vanessacs
dc.contributor.authorMichlovská, Lenkacs
dc.contributor.authorVištejnová, Luciecs
dc.contributor.authorZavaďáková, Annacs
dc.contributor.authorŠmerková, Kristýnacs
dc.contributor.authorKočiová, Silviacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorVojtová, Lucycs
dc.coverage.issue1cs
dc.coverage.volume19cs
dc.date.issued2021-12-01cs
dc.description.abstractIn a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of the biocompatibility and risk of exposure to nanomaterials is limited. Here, in vitro and ex ovo biocompatibility of naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified with thermostable fibroblast growth factor 2 (FGF2-STAB®), to enhance healing and selenium nanoparticles (SeNPs) to provide antibacterial activity, were evaluated. Biocompatibility and cytotoxicity were tested in vitro using normal human dermal fibroblasts (NHDF) with scaffolds and SeNPs and FGF2-STAB® solutions. Metabolic activity assays indicated an antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs. The half-maximal inhibitory concentration (IC50) of SeNPs for NHDF was 18.9 µg/ml and IC80 was 5.6 µg/ml. The angiogenic properties of the scaffolds were monitored ex ovo using a chick chorioallantoic membrane (CAM) assay and the cytotoxicity of SeNPs over IC80 value was confirmed. Furthermore, the positive effect of FGF2-STAB® at very low concentrations (0.01 µg/ml) on NHDF metabolic activity was observed. Based on detailed in vitro testing, the optimal concentrations of additives in the scaffolds were determined, specifically 1 µg/ml of FGF2-STAB® and 1 µg/ml of SeNPs. The scaffolds were further subjected to antimicrobial tests, where an increase in selenium concentration in the collagen/chitosan scaffolds increased the antibacterial activity. This work highlights the antimicrobial ability and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® and SeNPs. Moreover, we suggest that these sponges could be used as scaffolds for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. Due to their antimicrobial properties, these scaffolds are also highly promising for tissue replacement requiring the prevention of infection.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Nanobiotechnology. 2021, vol. 19, issue 1, p. 1-16.en
dc.identifier.doi10.1186/s12951-021-00849-wcs
dc.identifier.issn1477-3155cs
dc.identifier.orcid0000-0003-1683-5144cs
dc.identifier.orcid0000-0002-7963-1715cs
dc.identifier.orcid0000-0002-1667-7660cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.other171605cs
dc.identifier.researcheridB-1133-2014cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.scopus25621760100cs
dc.identifier.scopus6603604023cs
dc.identifier.scopus12039667200cs
dc.identifier.urihttp://hdl.handle.net/11012/196773
dc.language.isoencs
dc.publisherBioMed Central Ltdcs
dc.relation.ispartofJournal of Nanobiotechnologycs
dc.relation.urihttps://jnanobiotechnology.biomedcentral.com/track/pdf/10.1186/s12951-021-00849-w.pdfcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1477-3155/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subject3D porous scaffolden
dc.subjectChitosanen
dc.subjectCollagenen
dc.subjectFibroblast growth factor 2en
dc.subjectSelenium nanoparticlesen
dc.titleMutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-171605en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:05en
sync.item.modts2025.01.17 19:35:46en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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