but.defence	Studentka Klaudia Mesíková prezentovala svou diplomovou práci a zodpověděla dotazy v průběhu diskuze. Studentka svou práci úspěšně obhájila.	cs
but.jazyk	angličtina (English)
but.program	Bioengineering	cs
but.result	práce byla úspěšně obhájena	cs
dc.contributor.advisor	Paštěka, Richard	en
dc.contributor.author	Mesíková, Klaudia	en
dc.contributor.referee	Forjan, Mathias	en
dc.date.accessioned	2022-07-02T06:54:33Z
dc.date.available	2022-07-02T06:54:33Z
dc.date.created	2022	cs
dc.description.abstract	A mechanical combined lung model is a type of model used in human breathing simulation. The biggest currency of the model is a high similarity with the human lungs. In order to work with the animal lungs for a longer time and so follow the principles of the 3Rs, a perfusion system is involved in the procedure. The perfusion system filled with a chosen perfusate solution is responsible to prolong the period in which the animal lungs are viable for experiments and storage in the ex-vivo environment. The development of the properly functioning perfusion system is based on the several components included in the process. Choosing the right solution for the perfusion of the inner environment of the lungs is one of the most important things that need to be taken into account. The roller pump is considered the drive motor of the system. Pressure and flow sensors are responsible for monitoring the process parameters that could describe the functionality and the ability to preserve the animal lungs in the ex-vivo environment. The validation of the developed system by using the fresh animal lungs is a part of the thesis as well as the checking procedure of the solution’s influence with the time of the storage. The perfusion system was successfully created and tested. The pressure and flow parameters gained during the measurement were compared while using the saline solution, the Ringer’s solution, and Histofix in the system. The compliance parameter of the lungs were been monitored during the perfusion as well as during the storage with the aim to determine the behaviour of the preserved lungs with the time and the impact of the chosen solution on it. Compliance initially decreased and then stabilized at a certain value throughout the storage period. For the perfusion with the saline and Ringer’s solution, it dropped by one-third. For Histofix preservation, the drop was by half of the initial compliance. The preservation time without the presence of the tissue necrosis was 120 hours using the Saline solution, 240 hours using the Ringer’s solution, and at least 268 hours using Histofix. The perfusion system could further be used in medical research and make a positive aspect in terms of less consumption of the animal organs for experimental purposes in various fields of the research. For future research, the improvement of the perfusion system and solution composition to ensure even longer preservation is welcomed.	en
dc.description.abstract	A mechanical combined lung model is a type of model used in human breathing simulation. The biggest currency of the model is a high similarity with the human lungs. In order to work with the animal lungs for a longer time and so follow the principles of the 3Rs, a perfusion system is involved in the procedure. The perfusion system filled with a chosen perfusate solution is responsible to prolong the period in which the animal lungs are viable for experiments and storage in the ex-vivo environment. The development of the properly functioning perfusion system is based on the several components included in the process. Choosing the right solution for the perfusion of the inner environment of the lungs is one of the most important things that need to be taken into account. The roller pump is considered the drive motor of the system. Pressure and flow sensors are responsible for monitoring the process parameters that could describe the functionality and the ability to preserve the animal lungs in the ex-vivo environment. The validation of the developed system by using the fresh animal lungs is a part of the thesis as well as the checking procedure of the solution’s influence with the time of the storage. The perfusion system was successfully created and tested. The pressure and flow parameters gained during the measurement were compared while using the saline solution, the Ringer’s solution, and Histofix in the system. The compliance parameter of the lungs were been monitored during the perfusion as well as during the storage with the aim to determine the behaviour of the preserved lungs with the time and the impact of the chosen solution on it. Compliance initially decreased and then stabilized at a certain value throughout the storage period. For the perfusion with the saline and Ringer’s solution, it dropped by one-third. For Histofix preservation, the drop was by half of the initial compliance. The preservation time without the presence of the tissue necrosis was 120 hours using the Saline solution, 240 hours using the Ringer’s solution, and at least 268 hours using Histofix. The perfusion system could further be used in medical research and make a positive aspect in terms of less consumption of the animal organs for experimental purposes in various fields of the research. For future research, the improvement of the perfusion system and solution composition to ensure even longer preservation is welcomed.	cs
dc.description.mark	A	cs
dc.identifier.citation	MESÍKOVÁ, K. Vývoj ex vivo plicního perfuzního systému pro animální modely [online]. Brno: Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. 2022.	cs
dc.identifier.other	137500	cs
dc.identifier.uri	http://hdl.handle.net/11012/208147
dc.language.iso	en	cs
dc.publisher	Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií	cs
dc.rights	Standardní licenční smlouva - přístup k plnému textu bez omezení	cs
dc.subject	Perfusate solution	en
dc.subject	Perfusion system	en
dc.subject	Animal lungs	en
dc.subject	Preservation	en
dc.subject	Microcontroller	en
dc.subject	Perfusate solution	cs
dc.subject	Perfusion system	cs
dc.subject	Animal lungs	cs
dc.subject	Preservation	cs
dc.subject	Microcontroller	cs
dc.title	Vývoj ex vivo plicního perfuzního systému pro animální modely	en
dc.title.alternative	Development of an ex vivo lung perfusion system focusing on the preservation of fresh animal lungs for experiments and storage	cs
dc.type	Text	cs
dc.type.driver	masterThesis	en
dc.type.evskp	diplomová práce	cs
dcterms.dateAccepted	2022-06-21	cs
dcterms.modified	2022-07-01-13:35:46	cs
eprints.affiliatedInstitution.faculty	Fakulta elektrotechniky a komunikačních technologií	cs
sync.item.dbid	137500	en
sync.item.dbtype	ZP	en
sync.item.insts	2022.07.02 08:54:32	en
sync.item.modts	2022.07.02 08:13:46	en
thesis.discipline	bez specializace	cs
thesis.grantor	Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrství	cs
thesis.level	Inženýrský	cs
thesis.name	Ing.	cs

Vývoj ex vivo plicního perfuzního systému pro animální modely

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