Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery

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dc.contributor.authorKadlecová, Zuzanacs
dc.contributor.authorSevriugina, Veronikacs
dc.contributor.authorLysáková, Kláracs
dc.contributor.authorRychetský, Matějcs
dc.contributor.authorChamradová, Ivanacs
dc.contributor.authorVojtová, Lucycs
dc.coverage.issue1cs
dc.coverage.volume25cs
dc.date.accessioned2024-02-23T12:46:04Z
dc.date.available2024-02-23T12:46:04Z
dc.date.issued2023-12-22cs
dc.description.abstractFat grafting, a key regenerative medicine technique, often requires repeat procedures due to high-fat reabsorption and volume loss. Addressing this, a novel drug delivery system uniquely combines a thermosensitive, FDA-approved hydrogel (itaconic acid-modified PLGA-PEG-PLGA copolymer) with FGF2-STAB, a stable fibroblast growth factor 2 with a 21-day stability, far exceeding a few hours of wild-type FGF2's stability. Additionally, the growth factor was encapsulated in "green" liposomes prepared via the Mozafari method, ensuring pH protection. The system, characterized by first-order FGF2-STAB release, employs green chemistry for biocompatibility, bioactivity, and eco-friendliness. The liposomes, with diameters of 85.73 +/- 3.85 nm and 68.6 +/- 2.2% encapsulation efficiency, allowed controlled FGF2-STAB release from the hydrogel compared to the unencapsulated FGF2-STAB. Yet, the protein compromised the carrier's hydrolytic stability. Prior tests were conducted on model proteins human albumin (efficiency 80.8 +/- 3.2%) and lysozyme (efficiency 81.0 +/- 2.7%). This injectable thermosensitive system could advance reconstructive medicine and cosmetic procedures.en
dc.formattextcs
dc.format.extent67-76cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBIOMACROMOLECULES. 2023, vol. 25, issue 1, p. 67-76.en
dc.identifier.doi10.1021/acs.biomac.3c00736cs
dc.identifier.issn1525-7797cs
dc.identifier.orcid0000-0003-1267-8647cs
dc.identifier.orcid0009-0009-6888-9567cs
dc.identifier.orcid0000-0001-7927-946Xcs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.other187107cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.scopus12039667200cs
dc.identifier.urihttps://hdl.handle.net/11012/245202
dc.language.isoencs
dc.publisherAMER CHEMICAL SOCcs
dc.relation.ispartofBIOMACROMOLECULEScs
dc.relation.urihttps://pubs.acs.org/doi/epdf/10.1021/acs.biomac.3c00736cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1525-7797/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectDrug deliveryen
dc.subjectPLGA-PEG-PLGA copolymeren
dc.subjectitaconic aciden
dc.subjectFGF2-STAB®en
dc.subjectMozafari methoden
dc.subjectliposomesen
dc.titleLiposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Deliveryen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-187107en
sync.item.dbtypeVAVen
sync.item.insts2024.02.23 13:46:04en
sync.item.modts2024.02.23 13:13:40en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé polymerní materiály a kompozitcs
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