Liposomes Affect Protein Release and Stability of ITA-Modified PLGA-PEG-PLGA Hydrogel Carriers for Controlled Drug Delivery
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Date
2023-12-22
Authors
Kadlecová, Zuzana
Sevriugina, Veronika
Tušlová, Klára
Rychetský, Matěj
Chamradová, Ivana
Vojtová, Lucy
0000-0003-1267-8647Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
AMER CHEMICAL SOC
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Abstract
Fat grafting, a key regenerative medicine technique, often requires repeat procedures due to high-fat reabsorption and volume loss. Addressing this, a novel drug delivery system uniquely combines a thermosensitive, FDA-approved hydrogel (itaconic acid-modified PLGA-PEG-PLGA copolymer) with FGF2-STAB, a stable fibroblast growth factor 2 with a 21-day stability, far exceeding a few hours of wild-type FGF2's stability. Additionally, the growth factor was encapsulated in "green" liposomes prepared via the Mozafari method, ensuring pH protection. The system, characterized by first-order FGF2-STAB release, employs green chemistry for biocompatibility, bioactivity, and eco-friendliness. The liposomes, with diameters of 85.73 +/- 3.85 nm and 68.6 +/- 2.2% encapsulation efficiency, allowed controlled FGF2-STAB release from the hydrogel compared to the unencapsulated FGF2-STAB. Yet, the protein compromised the carrier's hydrolytic stability. Prior tests were conducted on model proteins human albumin (efficiency 80.8 +/- 3.2%) and lysozyme (efficiency 81.0 +/- 2.7%). This injectable thermosensitive system could advance reconstructive medicine and cosmetic procedures.
Description
Citation
BIOMACROMOLECULES. 2023, vol. 25, issue 1, p. 67-76.
https://pubs.acs.org/doi/epdf/10.1021/acs.biomac.3c00736
https://pubs.acs.org/doi/epdf/10.1021/acs.biomac.3c00736
Document type
Peer-reviewed
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Published version
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Language of document
en