Pilot-scale production of poly--hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactor

Simple item page
dc.contributor.authorTroschl, Clemenscs
dc.contributor.authorMeixner, Katharinacs
dc.contributor.authorFritz, Inescs
dc.contributor.authorLeitner, Klauscs
dc.contributor.authorPalacios Romero, Patricia Alejandracs
dc.contributor.authorKovalčík, Adriánacs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorDrosg, Bernhardcs
dc.coverage.issue1cs
dc.coverage.volume34cs
dc.date.issued2018-07-20cs
dc.description.abstractThe biopolymer poly--hydroxybutyrate (PHB) can be used as a promising bioplastic. It has a broad range of applications and is degraded relatively rapidly by soil organisms. Like many prokaryotes, the cyanobacterium Synechocystis sp. CCALA192 produces this biopolymer as a storage compound, especially under nutrient limitation. In a 200-L tubular photobioreactor, we cultivated Synechocystis sp. CCALA192 semi-continuously over a period of 75days with CO2 as sole carbon source. A two-stage cultivation strategy was performed, where after 5–7days nitrogen was depleted and the culture started to produce PHB and gradually turned from blue-green to yellow. After 16–20days, 90% of the culture were harvested and the residual 10% were used as inoculum for the following cycle. The harvested culture had an average biomass concentration of 1.0g/L with an average PHB content of 12.5% of cell dry weight. After restarting with fresh nutrients, the yellow culture turned blue-green again and degraded the PHB within 24–48h. When nitrogen of the medium was consumed, PHB was produced again and the cycle continued. In the late stage of each production cycle, a ripening process was observed, where no CO2 was consumed but the PHB concentration was still rising at the expense of the existing glycogen rich biomass. Establishing a stable Synechocystis sp. CCALA192 culture under non-sterile conditions turned out to be difficult, as this small unicellular organism is very sensitive and easily grazed by protozoa. Therefore, a special cultivation strategy with partially anoxic conditions was necessary.en
dc.formattextcs
dc.format.extent116-125cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationAlgal Research. 2018, vol. 34, issue 1, p. 116-125.en
dc.identifier.doi10.1016/j.algal.2018.07.011cs
dc.identifier.issn2211-9264cs
dc.identifier.orcid0000-0003-4833-7369cs
dc.identifier.orcid0000-0002-6211-9643cs
dc.identifier.other150270cs
dc.identifier.researcheridI-7386-2015cs
dc.identifier.scopus25621752900cs
dc.identifier.urihttp://hdl.handle.net/11012/194778
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofAlgal Researchcs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2211926418302923cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2211-9264/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectCyanobacteriaen
dc.subjectSynechocystis sp. CCALA192en
dc.subjectPHBen
dc.subjectTubular photobioreactoren
dc.titlePilot-scale production of poly--hydroxybutyrate with the cyanobacterium Synechocytis sp. CCALA192 in a non-sterile tubular photobioreactoren
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-150270en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:37:55en
sync.item.modts2025.01.17 18:49:35en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Pilotscale_paper_final_draft.pdf
Size:
1006.48 KB
Format:
Adobe Portable Document Format
Description:
Pilotscale_paper_final_draft.pdf
Download
Collections
Ústav chemie potravin a biotechnologií
Centrum materiálového výzkumu