Determination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

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dc.contributor.authorFrangu, Arbërcs
dc.contributor.authorAmirmansoor, Ashrafics
dc.contributor.authorSýs, Milancs
dc.contributor.authorArbneshi, Tahircs
dc.contributor.authorMetelka, Radovancs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorVlček, Milancs
dc.contributor.authorRichtera, Lukášcs
dc.coverage.issue7cs
dc.coverage.volume10cs
dc.date.accessioned2020-08-18T10:57:50Z
dc.date.available2020-08-18T10:57:50Z
dc.date.issued2020-04-30cs
dc.description.abstractIn this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion((R)) layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationApplied Sciences - Basel. 2020, vol. 10, issue 7, p. 1-13.en
dc.identifier.doi10.3390/app10072497cs
dc.identifier.issn2076-3417cs
dc.identifier.other164381cs
dc.identifier.urihttp://hdl.handle.net/11012/194794
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofApplied Sciences - Baselcs
dc.relation.urihttps://www.mdpi.com/2076-3417/10/7/2497cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-3417/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectamperometryen
dc.subjectascorbic aciden
dc.subjectberriesen
dc.subjectbiosensoren
dc.subjectmulti-wall carbon nanotubesen
dc.subjectcarbon paste electrodeen
dc.subjecttyrosinaseen
dc.subjectTroloxen
dc.subjectantioxidant capacityen
dc.titleDetermination of trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164381en
sync.item.dbtypeVAVen
sync.item.insts2020.12.07 12:55:15en
sync.item.modts2020.12.07 12:14:37en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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