Polyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization.

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dc.contributor.authorKrajčovič, Tomášcs
dc.contributor.authorBučko, Marekcs
dc.contributor.authorVikartovská, Alicacs
dc.contributor.authorLacík, Igorcs
dc.contributor.authorUhelská, Luciacs
dc.contributor.authorChorvát, Dušancs
dc.contributor.authorNeděla, Vilémcs
dc.contributor.authorTihlaříková, Evacs
dc.contributor.authorGericke, Martincs
dc.contributor.authorHeinze, Thomas S.cs
dc.contributor.authorGemeiner, Petercs
dc.coverage.issue11cs
dc.coverage.volume7cs
dc.date.accessioned2020-11-25T15:54:33Z
dc.date.available2020-11-25T15:54:33Z
dc.date.issued2017-11-21cs
dc.description.abstractA novel immobilization matrix for the entrapment of viable whole-cell Baeyer–Villiger monooxygenase was developed. Viable recombinant Escherichia coli cells overexpressing cyclohexanone monooxygenase were entrapped in polyelectrolyte complex beads prepared by a two-step reaction of oppositely-charged polymers including highly defined cellulose sulphate. Immobilized cells exhibited higher operational stability than free cells during 10 repeated cycles of Baeyer–Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to the corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one. The morphology of polyelectrolyte complex beads was characterised by environmental scanning electron microscopy; the spatial distribution of polymers in the beads and cell viability were examined using confocal laser scanning microscopy, and the texture was characterised by the mechanical resistance measurements.en
dc.formattextcs
dc.format.extent353-364cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationCatalysts. 2017, vol. 7, issue 11, p. 353-364.en
dc.identifier.doi10.3390/catal7110353cs
dc.identifier.issn2073-4344cs
dc.identifier.other158726cs
dc.identifier.urihttp://hdl.handle.net/11012/195685
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofCatalystscs
dc.relation.urihttps://www.mdpi.com/2073-4344/7/11/353cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4344/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyelectrolyte complex beads * environmental scanning electron microscopy * confocal laser scanning microscopy * Baeyer-Villiger biooxidation * cyclohexanone monoxygenase * immobilization * viable whole-cell biocatalysten
dc.titlePolyelectrolyte Complex Beads by Novel Two-Step Process for Improved Performance of Viable Whole-Cell Baeyer-Villiger Monoxygenase by Immobilization.en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158726en
sync.item.dbtypeVAVen
sync.item.insts2020.11.25 16:54:33en
sync.item.modts2020.11.25 16:14:23en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav elektrotechnologiecs
thesis.grantorVysoké učení technické v Brně. . Ústav přístrojové techniky AV ČRcs
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