Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

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dc.contributor.authorAmirmansoor, Ashrafics
dc.contributor.authorSýs, Milancs
dc.contributor.authorSedláčková, Eliškacs
dc.contributor.authorFarag, Amir Shaabancs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorPřibyl, Jancs
dc.contributor.authorRichtera, Lukášcs
dc.coverage.issue13cs
dc.coverage.volume19cs
dc.date.accessioned2020-08-04T11:03:45Z
dc.date.available2020-08-04T11:03:45Z
dc.date.issued2019-07-03cs
dc.description.abstractThe inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2019, vol. 19, issue 13, p. 1-15.en
dc.identifier.doi10.3390/s19132939cs
dc.identifier.issn1424-8220cs
dc.identifier.other158050cs
dc.identifier.urihttp://hdl.handle.net/11012/180521
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttps://www.mdpi.com/1424-8220/19/13/2939cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectglucose oxidaseen
dc.subjectheavy metalsen
dc.subjectamperometric biosensoren
dc.subjectnon-competitive inhibitionen
dc.titleApplication of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metalsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158050en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:45en
sync.item.modts2020.08.04 12:18:06en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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