Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay

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dc.contributor.authorSedláčková, Eliškacs
dc.contributor.authorBytešníková, Zuzanacs
dc.contributor.authorBirgusová, Eliškacs
dc.contributor.authorŠvec, Pavelcs
dc.contributor.authorAmirmansoor, Ashrafics
dc.contributor.authorEstrela, Pedrocs
dc.contributor.authorRichtera, Lukášcs
dc.coverage.issue21cs
dc.coverage.volume13cs
dc.date.accessioned2021-01-27T11:56:28Z
dc.date.available2021-01-27T11:56:28Z
dc.date.issued2020-11-30cs
dc.description.abstractThis work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)-gold (AuNPs), silver (AgNPs), and copper (CuNPs)-was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA-1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5 '-CCGG-3 ') islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U center dot mL(-1). Lastly, this study was enriched with the real serum sample, where a 0.19 U center dot mL(-1) LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMaterials . 2020, vol. 13, issue 21, p. 1-12.en
dc.identifier.doi10.3390/ma13214936cs
dc.identifier.issn1996-1944cs
dc.identifier.other168307cs
dc.identifier.urihttp://hdl.handle.net/11012/195920
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMaterialscs
dc.relation.urihttps://www.mdpi.com/1996-1944/13/21/4936cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1996-1944/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectnanomaterialsen
dc.subjectelectrochemical biosensoren
dc.subjectDNA methylationen
dc.subjectrGOen
dc.subjectbiomedical applicationsen
dc.titleLabel-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assayen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-168307en
sync.item.dbtypeVAVen
sync.item.insts2021.01.27 12:56:28en
sync.item.modts2021.01.27 12:15:51en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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