Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes

Abstract

Influenza viruses, as etiological agent of acute respiratory-related diseases, cause seasonal epidemics and/or less frequently pandemics with substantial mortality across the world. They display high mutation rates and complex evolutionary patterns inducing often significant resistance to antiviral drugs. We considered an electrochemical detection method, so called CombiMatrix ElectraSense core technology, to create one influenza array containing probes of all genes encodable by different subtypes of influenza type A genome. We detected particularly: neuraminidase, hemagglutinin, nucleocapsid protein, matrix protein 1, matrix protein 2, polymerase PB1, polymerase PA and nuclear export protein. Moreover, we succeeded to detect in vitro prepared mutations of the mentioned genes and it was found that the suggested array might be a suitable method not only for detection of the expression of the genes but also for detection of their mutationsInfluenza viruses, as etiological agent of acute respiratory-related diseases, cause seasonal epidemics and/or less frequently pandemics with substantial mortality across the world. They display high mutation rates and complex evolutionary patterns inducing often significant resistance to antiviral drugs. We considered an electrochemical detection method, so called CombiMatrix ElectraSense core technology, to create one influenza array containing probes of all genes encodable by different subtypes of influenza type A genome. We detected particularly: neuraminidase, hemagglutinin, nucleocapsid protein, matrix protein 1, matrix protein 2, polymerase PB1, polymerase PA and nuclear export protein. Moreover, we succeeded to detect in vitro prepared mutations of the mentioned genes and it was found that the suggested array might be a suitable method not only for detection of the expression of the genes but also for detection of their mutations