Use of micro computed-tomography and 3D printing for reverse engineering of mouse embryo nasal capsule

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Date
2016-03-02
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Mark
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IOP Publishing
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Abstract
Imaging of increasingly complex cartilage in vertebrate embryos is one of the key tasks of developmental biology. This is especially important to study shape-organizing processes during initial skeletal formation and growth. Advanced imaging techniques that are reflecting biological needs give a powerful impulse to push the boundaries of biological visualization. Recently, techniques for contrasting tissues and organs have improved considerably, extending traditional 2D imaging approaches to 3D. X-ray micro computed tomography (uCT), which allows 3D imaging of biological objects including their internal structures with a resolution in the micrometer range, in combination with contrasting techniques seems to be the most suitable approach for non-destructive imaging of embryonic developing cartilage. Despite there are many software-based ways for visualization of 3D data sets, having a real solid model of the studied object might give novel opportunities to fully understand the shape-organizing processes in the developing body. In this feasibility study we demonstrated the full procedure of creating a real 3D object of mouse embryo nasal capsule, i.e. the staining, the CT scanning combined by the advanced data processing and the 3D printing.
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Citation
Journal of Instrumentation. 2016, vol. 11, issue 3, p. 1-11.
http://iopscience.iop.org/article/10.1088/1748-0221/11/03/C03006
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Peer-reviewed
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en
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Creative Commons Attribution 3.0 Unported
http://creativecommons.org/licenses/by/3.0/
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