G-quadruplexes in H1N1 influenza genomes

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dc.contributor.authorBrázda, Václavcs
dc.contributor.authorPorubiaková, Otíliacs
dc.contributor.authorCantara, Alessiocs
dc.contributor.authorBohálová, Natáliacs
dc.contributor.authorCoufal, Jancs
dc.contributor.authorBartas, Martincs
dc.contributor.authorFojta, Miroslavcs
dc.contributor.authorMergny, Jean-Louiscs
dc.coverage.issue1cs
dc.coverage.volume22cs
dc.date.issued2021-01-24cs
dc.description.abstractBackground: Influenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment. Results: PQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000 nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS; even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods. Conclusions: The results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBMC GENOMICS. 2021, vol. 22, issue 1, p. 1-11.en
dc.identifier.doi10.1186/s12864-021-07377-9cs
dc.identifier.issn1471-2164cs
dc.identifier.orcid0000-0003-2837-4226cs
dc.identifier.orcid0000-0001-9940-0941cs
dc.identifier.other168095cs
dc.identifier.researcheridF-9582-2011cs
dc.identifier.urihttp://hdl.handle.net/11012/196699
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofBMC GENOMICScs
dc.relation.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1471-2164/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectInfluenza virusen
dc.subjectG-quadruplexen
dc.subjectG4Hunteren
dc.titleG-quadruplexes in H1N1 influenza genomesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-168095en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:04en
sync.item.modts2025.01.17 20:33:17en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Fakulta chemickács
thesis.grantorVysoké učení technické v Brně. . Akademie věd ČRcs
thesis.grantorVysoké učení technické v Brně. . Masarykova Univerzita v Brněcs
thesis.grantorVysoké učení technické v Brně. . Ostravská univerzita v Ostravěcs
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