G-quadruplexes in H1N1 influenza genomes
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Date
2021-01-24
Authors
Brázda, Václav
Porubiaková, Otília
Cantara, Alessio
Bohálová, Natália
Coufal, Jan
Bartas, Martin
Fojta, Miroslav
Mergny, Jean-Louis
0000-0003-2837-4226Advisor
Referee
Mark
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Publisher
Springer Nature
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Abstract
Background: Influenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment. Results: PQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000 nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS; even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods. Conclusions: The results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets.
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BMC GENOMICS. 2021, vol. 22, issue 1, p. 1-11.
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9
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Peer-reviewed
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Published version
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en