CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing

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dc.contributor.authorSur, Vishma Pratapcs
dc.contributor.authorKomínková, Markétacs
dc.contributor.authorBuchtová, Žanetacs
dc.contributor.authorDoleželíková, Kristýnacs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorMoulick, Amitavacs
dc.coverage.issue10cs
dc.coverage.volume9cs
dc.date.issued2019-10-31cs
dc.description.abstractThe physical and chemical synthesis methods of quantum dots (QDs) are generally unfavorable for biological applications. To overcome this limitation, the development of a novel "green" route to produce highly-fluorescent CdSe QDs constitutes a promising substitute approach. In the present work, CdSe QDs were biosynthesized in yeast Saccharomyces cerevisiae using a novel method, where we showed for the first time that the concentration of tryptone highly affects the synthesis process. The optimum concentration of tryptone was found to be 25 g/L for the highest yield. Different methods were used to optimize the QD extraction from yeast, and the best method was found to be by denaturation at 80 degrees C along with an ultrasound needle. Multiple physical characterizations including transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and spectrophotometry confirmed the optical features size and shape distribution of the QDs. We showed that the novel conjugate of the CdSe QDs and a cell-penetrating peptide (hecate) can detect bacterial cells very efficiently under a fluorescent microscope. The conjugate also showed strong antibacterial activity against vancomycin-resistant Staphylococcus aureus (VRSA), methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli, which may help us to cope with the problem of rising antibiotic resistance.en
dc.description.abstractThe physical and chemical synthesis methods of quantum dots (QDs) are generally unfavorable for biological applications. To overcome this limitation, the development of a novel "green" route to produce highly-fluorescent CdSe QDs constitutes a promising substitute approach. In the present work, CdSe QDs were biosynthesized in yeast Saccharomyces cerevisiae using a novel method, where we showed for the first time that the concentration of tryptone highly affects the synthesis process. The optimum concentration of tryptone was found to be 25 g/L for the highest yield. Different methods were used to optimize the QD extraction from yeast, and the best method was found to be by denaturation at 80 degrees C along with an ultrasound needle. Multiple physical characterizations including transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), and spectrophotometry confirmed the optical features size and shape distribution of the QDs. We showed that the novel conjugate of the CdSe QDs and a cell-penetrating peptide (hecate) can detect bacterial cells very efficiently under a fluorescent microscope. The conjugate also showed strong antibacterial activity against vancomycin-resistant Staphylococcus aureus (VRSA), methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli, which may help us to cope with the problem of rising antibiotic resistance.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNanomaterials. 2019, vol. 9, issue 10, p. 1-15.en
dc.identifier.doi10.3390/nano9101463cs
dc.identifier.issn2079-4991cs
dc.identifier.orcid0000-0002-1175-7194cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.other160566cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.scopus14012648400cs
dc.identifier.scopus55783172600cs
dc.identifier.urihttp://hdl.handle.net/11012/184080
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofNanomaterialscs
dc.relation.urihttps://www.mdpi.com/2079-4991/9/10/1463cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2079-4991/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbiosynthesisen
dc.subjecttryptoneen
dc.subjectQDsen
dc.subjectcell-penetrating peptideen
dc.subjectantibacterialen
dc.subjectbiosynthesis
dc.subjecttryptone
dc.subjectQDs
dc.subjectcell-penetrating peptide
dc.subjectantibacterial
dc.titleCdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killingen
dc.title.alternativeCdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-160566en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:16:31en
sync.item.modts2025.10.14 09:47:13en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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