Lactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detector

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dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorHubálek, Jaromírcs
dc.contributor.authorKřížková, Soňacs
dc.contributor.authorTrnková, Libušecs
dc.contributor.authorKizek, Renécs
dc.coverage.issue8cs
dc.coverage.volume2008cs
dc.date.accessioned2017-10-12T10:46:07Z
dc.date.available2017-10-12T10:46:07Z
dc.date.issued2008-01-24cs
dc.description.abstractLactoferrin is a multifunctional protein with antimicrobial activity and others to health beneficial properties. The main aim of this work was to propose easy to use technique for lactoferrin isolation from cow colostrum samples. Primarily we utilized sodium dodecyl sulphate-polyacrylamide gel electrophoresis for isolation of lactoferrin from the real samples. Moreover we tested automated microfluidic Experion electrophoresis system to isolate lactoferrin from the collostrum sample. The well developed signal of lactoferrin was determined with detection limit (3 S/N) of 20 ng/ml. In spite of the fact that Experion is faster than SDS-PAGE both separation techniques cannot be used in routine analysis. Therefore we have tested third separation technique, ion exchange chromatography, using monolithic column coupled with UV-VIS detector (LCUV-VIS). We optimized wave length (280 nm), ionic strength of the elution solution (1.5 M NaCl) and flow rate of the retention and elution solutions (0.25 ml/min and 0.75 ml/min. respectively).en
dc.formattextcs
dc.format.extent464-487cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2008, vol. 2008, issue 8, p. 464-487.en
dc.identifier.doi10.3390/s8010464cs
dc.identifier.issn1424-8220cs
dc.identifier.other49158cs
dc.identifier.urihttp://hdl.handle.net/11012/70078
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttp://www.mdpi.com/1424-8220/8/1/464cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectLactoferrinen
dc.subjectMilk Proteinen
dc.subjectLinear Sweep Voltammetryen
dc.subjectScreen-Printed Carbon Electrodeen
dc.subjectElectrochemistryen
dc.subjectLiquid Chromatographyen
dc.subjectMonolithic Columnen
dc.subjectUV-VIS Spectrometryen
dc.titleLactoferrin Isolation Using Monolithic Column Coupled with Spectrometric or Micro-Amperometric Detectoren
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-49158en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 10:59:52en
sync.item.modts2020.04.01 05:58:12en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
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