Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

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dc.contributor.authorSkaličková, Sylviecs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorRuttkay-Nedecký, Branislavcs
dc.contributor.authorJimenez Jimenez, Ana Mariacs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorKremplová, Monikacs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorStiborová, Mariecs
dc.contributor.authorEckschlager, Tomášcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue3cs
dc.coverage.volume16cs
dc.date.issued2016-03-01cs
dc.description.abstractLiposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg•mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.en
dc.description.abstractLiposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg•mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2016, vol. 16, issue 3, p. 1-11.en
dc.identifier.doi10.3390/s16030290cs
dc.identifier.issn1424-8220cs
dc.identifier.orcid0000-0001-6332-8917cs
dc.identifier.orcid0000-0003-1736-492Xcs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other128498cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus6603604023cs
dc.identifier.urihttp://hdl.handle.net/11012/63728
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttp://www.mdpi.com/1424-8220/16/3/290cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectliposomeen
dc.subjectgold nanoparticlesen
dc.subjectN-mycen
dc.subjectdoxorubicinen
dc.subjectellipticineen
dc.subjectetoposideen
dc.subjectliposome
dc.subjectgold nanoparticles
dc.subjectN-myc
dc.subjectdoxorubicin
dc.subjectellipticine
dc.subjectetoposide
dc.titleFluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)en
dc.title.alternativeFluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-128498en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:17:07en
sync.item.modts2025.10.14 10:19:22en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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