Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure

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dc.contributor.authorHorký, Pavelcs
dc.contributor.authorSkaličková, Sylviecs
dc.contributor.authorUrbánková, Lenkacs
dc.contributor.authorBaholet, Dariacs
dc.contributor.authorKočiová, Silviacs
dc.contributor.authorBytešníková, Zuzanacs
dc.contributor.authorKabourková, Eliškacs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorGagić, Milicacs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorSmolíková, Vendulacs
dc.contributor.authorVáclavková, Evacs
dc.contributor.authorNevrkla, Pavelcs
dc.contributor.authorKnot, Pavelcs
dc.contributor.authorKryštofová, Olgacs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorSkládanka, Jiřícs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorŠmerková, Kristýnacs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2019-02-12cs
dc.description.abstractBackground: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Results: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6mmol/L), followed by Escherichia coli (IC50 0.8-1.5mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2-4.7mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P<0.05) Zn level in liver and kidney of all treated groups was found, nevertheless ZnNPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P<0.05) in all zinc groups after 30d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P<0.05). Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Animal Science and Biotechnology. 2019, vol. 10, issue 1, p. 1-12.en
dc.identifier.doi10.1186/s40104-019-0319-8cs
dc.identifier.issn2049-1891cs
dc.identifier.orcid0000-0003-4254-5992cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0002-6851-6332cs
dc.identifier.orcid0000-0003-4122-0694cs
dc.identifier.orcid0000-0001-5404-9553cs
dc.identifier.orcid0000-0002-4575-3562cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-1667-7660cs
dc.identifier.other156204cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridP-3551-2018cs
dc.identifier.researcheridH-8903-2018cs
dc.identifier.researcheridE-1127-2012cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus56115133500cs
dc.identifier.scopus18234063900cs
dc.identifier.scopus6603604023cs
dc.identifier.urihttp://hdl.handle.net/11012/138317
dc.language.isoencs
dc.publisherBioMed Centralcs
dc.relation.ispartofJournal of Animal Science and Biotechnologycs
dc.relation.urihttp://link.springer.com/article/10.1186/s40104-019-0319-8cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2049-1891/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectAerobic bacteriaen
dc.subjectAntibioticsen
dc.subjectColiformsen
dc.subjectNanomaterialsen
dc.subjectOxidative stressen
dc.titleZinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposureen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156204en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:47en
sync.item.modts2025.01.17 16:44:55en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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