The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

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dc.contributor.authorMelčová, Veronikacs
dc.contributor.authorKrobot, Štěpáncs
dc.contributor.authorŠindelář, Jancs
dc.contributor.authorŠebová, Evacs
dc.contributor.authorKlusáček Rampichová, Michalacs
dc.contributor.authorPřikryl, Radekcs
dc.coverage.issueAugustcs
dc.coverage.volume16cs
dc.date.accessioned2024-12-10T13:55:22Z
dc.date.available2024-12-10T13:55:22Z
dc.date.issued2024-08-01cs
dc.description.abstractThe aim of this work is to explore the effect of surface characteristics of the 3D printed scaffolds on their suitability for Saos-2 cells growth. Two promising materials for bone tissue engineering were examined, both containing poly(3-hydroxybutyrate)/poly(d,l-lactide) plasticized blend and one of them filled with bioactive tricalcium phosphate. The surface free energy (SFE) of samples was determined by contact angle measurement with four liquids. For non-filled sample the water contact angle (WCA) was 74° and its SFE was 40 mNm1. The composite sample exhibited substantially decreased WCA, 61°. Moreover, the addition of the tricalcium phosphate caused doubling of the polar component of the SFE which increased by a total of 13%. 3D printed surfaces prepared by fused deposition modeling method showed a profound increase of WCA due to the increase in their surface roughness which was analyzed by confocal microscopy. The change in wetting properties strongly affected the behavior of Saos-2 cells on printed surfaces. The number of cells as measured by DNA quantification linearly decreased with increasing surface WCA. The highest number of cells was observed on the pressed samples and 3D printed surfaces made of simple lines and a grid with 50 m gap between lines. The favored surfaces exhibit WCA under 80° which is important information for the future design of the scaffolds.en
dc.formattextcs
dc.format.extent1-18cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationResults in Surfaces and Interfaces. 2024, vol. 16, issue August, p. 1-18.en
dc.identifier.doi10.1016/j.rsurfi.2024.100271cs
dc.identifier.issn2666-8459cs
dc.identifier.orcid0000-0002-2124-6953cs
dc.identifier.orcid0000-0002-8647-2097cs
dc.identifier.orcid0009-0001-5268-9327cs
dc.identifier.orcid0000-0002-7811-9840cs
dc.identifier.other189307cs
dc.identifier.researcheridABD-4360-2020cs
dc.identifier.researcheridHHN-0528-2022cs
dc.identifier.researcheridE-8210-2010cs
dc.identifier.scopus57204110910cs
dc.identifier.scopus57984865200cs
dc.identifier.urihttps://hdl.handle.net/11012/249739
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofResults in Surfaces and Interfacescs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihubcs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2666-8459/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectPoly(3-hydroxybutyrate)en
dc.subjectpolylactideen
dc.subject3D printingen
dc.subjectwater contact angleen
dc.subjectsurface energyen
dc.subjectsurface roughnessen
dc.titleThe effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfacesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-189307en
sync.item.dbtypeVAVen
sync.item.insts2024.12.10 14:55:22en
sync.item.modts2024.12.02 15:32:05en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie materiálůcs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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