The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

Melčová, Veronika; Krobot, Štěpán; Šindelář, Jan; Šebová, Eva; Klusáček Rampichová, Michala; Přikryl, Radek

The effect of surface roughness and wettability on the adhesion and proliferation of Saos-2 cells seeded on 3D printed poly(3-hydroxybutyrate)/polylactide (PHB/PLA) surfaces

Files

Results in surfaces and interfaces published article.pdf(12.02 MB)

Date

2024-08-01

Authors

Melčová, Veronika

Krobot, Štěpán

Šindelář, Jan

Šebová, Eva

Klusáček Rampichová, Michala

Přikryl, Radek

ORCID

Publisher

Elsevier

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Abstract

The aim of this work is to explore the effect of surface characteristics of the 3D printed scaffolds on their suitability for Saos-2 cells growth. Two promising materials for bone tissue engineering were examined, both containing poly(3-hydroxybutyrate)/poly(d,l-lactide) plasticized blend and one of them filled with bioactive tricalcium phosphate. The surface free energy (SFE) of samples was determined by contact angle measurement with four liquids. For non-filled sample the water contact angle (WCA) was 74° and its SFE was 40 mNm1. The composite sample exhibited substantially decreased WCA, 61°. Moreover, the addition of the tricalcium phosphate caused doubling of the polar component of the SFE which increased by a total of 13%. 3D printed surfaces prepared by fused deposition modeling method showed a profound increase of WCA due to the increase in their surface roughness which was analyzed by confocal microscopy. The change in wetting properties strongly affected the behavior of Saos-2 cells on printed surfaces. The number of cells as measured by DNA quantification linearly decreased with increasing surface WCA. The highest number of cells was observed on the pressed samples and 3D printed surfaces made of simple lines and a grid with 50 m gap between lines. The favored surfaces exhibit WCA under 80° which is important information for the future design of the scaffolds.

Keywords

Poly(3-hydroxybutyrate), polylactide, 3D printing, water contact angle, surface energy, surface roughness

Citation

Results in Surfaces and Interfaces. 2024, vol. 16, issue August, p. 1-18.
https://www.sciencedirect.com/science/article/pii/S2666845924000916?via%3Dihub

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/