Molecular imprinting technology for targeted analysis of proteins

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dc.contributor.authorHutařová, Jitkacs
dc.contributor.authorVaněčková, Terezacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorAdam, Vojtěchcs
dc.date.accessioned2018-08-23T10:52:55Z
dc.date.available2018-08-23T10:52:55Z
dc.date.issued2017-12-31cs
dc.description.abstractMolecular imprinting has appeared to be an effective technique for creating of selective recognition sites in synthetic polymers. This procedure comprises polymerization of monomer in a presence of target molecules (template). The subsequent template removal forms tailor-made cavities that are complementary in shape and size to the template molecules. For protein imprinting, the choice of the suitable polymers is limited and polymerization conditions need to be optimized. In our work, dopamine monomer was chosen for polymer formation due to its nontoxicity, ease of preparation and self-assembly. For the optimization of conditions, lysozyme with molecular weight 14.3 kDa was used and the functionality was evaluated by fluorimetry. Different concentration of dopamine and lysozyme for polymerization were tested. Under the optimized conditions, the limit of detection for lysozyme was found to be 7.8 µg/ml. Moreover, conditions for polymer formation for a purpose to reduce the overall time of analysis were investigated. The use of dopamine as a monomer in molecular imprinting shown to be beneficial in many aspects.en
dc.formattextcs
dc.format.extent873-877cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2017. 2017, p. 873-877.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.other148729cs
dc.identifier.urihttp://hdl.handle.net/11012/84189
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0098_Molecular-imprinting-technology-for-targeted-analysis-of-proteins.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectPolydopamineen
dc.subjectmolecularly imprinted polymeren
dc.subjectlysozymeen
dc.titleMolecular imprinting technology for targeted analysis of proteinsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-148729en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 11:01:49en
sync.item.modts2020.04.01 05:49:23en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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