Isolation of histamine using -Fe2O3 nanoparticles
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Date
2019-01-02
Authors
Gagić, Milica
Kopel, Pavel
Milosavljević, Vedran
Cernei, Natalia Vladimirovna
Zítka, Ondřej
Švec, Pavel
Jamroz, Ewelina
Adam, Vojtěch
Advisor
Referee
Mark
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Mendel University in Brno
Abstract
Histamine, biologically active amine, is normally present in the body and it is involved in a local regulation of physiological processes. It occurs in food as a product of microbial decarboxylation of the amino acid histidine, and the ingestion of foods that contain high levels of histamine can lead to poisoning. Hence, the presence of this biogenic amine is considered as an indicator of food spoilage. Many different methods are available to detect the presence of histamine in food samples. The aim of this study was to design a fast and low-cost method for histamine identification employing magnetic isolation and subsequent reaction of desorbed histamine with ninhydrin for final ion exchange chromatography quantification.
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Peer-reviewed
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en
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(C) Mendel University in Brno