An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents

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dc.contributor.authorPohanka, Miroslavcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue9cs
dc.coverage.volume13cs
dc.date.accessioned2020-08-04T11:03:40Z
dc.date.available2020-08-04T11:03:40Z
dc.date.issued2013-09-01cs
dc.description.abstractThe enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10(-12) mol/L for sarin, 6.31 x 10(-12) mol/L for soman, 6.17 x 10(-11) mol/L for tabun, and 2.19 x 10(-11) mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.en
dc.formattextcs
dc.format.extent11498-11506cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2013, vol. 13, issue 9, p. 11498-11506.en
dc.identifier.doi10.3390/s130911498cs
dc.identifier.issn1424-8220cs
dc.identifier.other143113cs
dc.identifier.urihttp://hdl.handle.net/11012/137241
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttps://www.mdpi.com/1424-8220/13/9/11498cs
dc.rightsCreative Commons Attribution 3.0 Unportedcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cs
dc.subjectbiosensoren
dc.subjectacetylcholinesteraseen
dc.subjectsarinen
dc.subjecttabunen
dc.subjectsomanen
dc.subjectVXen
dc.subjectinhibitoren
dc.subjectscreen printed electrodeen
dc.subjectvoltammetryen
dc.subjectamperometryen
dc.titleAn Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agentsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-143113en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:03:40en
sync.item.modts2020.08.04 12:19:12en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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