Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides

Simple item page
dc.contributor.authorVeselý, Radekcs
dc.contributor.authorJelínková, Pavlínacs
dc.contributor.authorHegerová, Dagmarcs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorKopel, Pavelcs
dc.contributor.authorMoulick, Amitavacs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorHeger, Zbyněkcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorZítka, Ondřejcs
dc.coverage.issue4cs
dc.coverage.volume9cs
dc.date.issued2016-04-01cs
dc.description.abstractThis paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential ( 9.1 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 0.4 to 33.1 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. 50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.en
dc.description.abstractThis paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential ( 9.1 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 0.4 to 33.1 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. 50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.en
dc.formattextcs
dc.format.extent260-1-260-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMaterials. 2016, vol. 9, issue 4, p. 260-1-260-15.en
dc.identifier.issn1996-1944cs
dc.identifier.orcid0000-0003-0826-234Xcs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0003-4216-9544cs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0002-3915-7270cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other126827cs
dc.identifier.researcheridJ-7030-2012cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridE-5711-2012cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridD-1973-2013cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus6603604023cs
dc.identifier.scopus55783172600cs
dc.identifier.scopus12040049600cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/63815
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMaterialscs
dc.relation.urihttp://dx.doi.org/10.3390/ma9040260cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1996-1944/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbranched chain amino acidsen
dc.subjectencapsulationen
dc.subjectEscherichia colien
dc.subjectnanomedicineen
dc.subjectStaphylococcus aureusen
dc.subjectbranched chain amino acids
dc.subjectencapsulation
dc.subjectEscherichia coli
dc.subjectnanomedicine
dc.subjectStaphylococcus aureus
dc.titleNanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptidesen
dc.title.alternativeNanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptidesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-126827en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:17:08en
sync.item.modts2025.10.14 09:53:51en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
materials0900260.pdf
Size:
2.78 MB
Format:
Adobe Portable Document Format
Description:
materials0900260.pdf
Download
Collections
Chytré nanonástroje