Hydroxyproline assay by HPLC-FLD applied for wound healing determination in rat model

Abstract

In this work, we focused on four types of oils and their response on wound healing of the skin of rats after dietary exposure. A set of samples consisted of 48 adult male rats of Wistar albino, which were divided into four groups according to the respective oil. The content of hydroxyproline in the skin tissue, used as a biomarker for wound healing, was determined by high-performance liquid chromatography with a fluorescence detector. There was found out, that the highest content of hydroxyproline was detected in the group of rats, which have been fed with the addition of palm oil (118.4 ± 1.9 µg/g). These results indicate that palm oil had the most positive effect on wound healing.In this work, we focused on four types of oils and their response on wound healing of the skin of rats after dietary exposure. A set of samples consisted of 48 adult male rats of Wistar albino, which were divided into four groups according to the respective oil. The content of hydroxyproline in the skin tissue, used as a biomarker for wound healing, was determined by high-performance liquid chromatography with a fluorescence detector. There was found out, that the highest content of hydroxyproline was detected in the group of rats, which have been fed with the addition of palm oil (118.4 ± 1.9 µg/g). These results indicate that palm oil had the most positive effect on wound healing.