Electrochemical biosensor based on modified reduced graphene oxide with silver nanoparticles for detection of methylated DNA

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dc.contributor.authorSedláčková, Eliškacs
dc.contributor.authorBirgusová, Eliškacs
dc.contributor.authorBytešníková, Zuzanacs
dc.contributor.authorŠvec, Pavelcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorAdam, Vojtěchcs
dc.date.accessioned2021-05-04T10:54:19Z
dc.date.available2021-05-04T10:54:19Z
dc.date.issued2020-06-10cs
dc.description.abstractDNA methylation is one of the most studied and basic epigenetic process related to several diseases such as diabetes, neurodegenerative or cardiovascular diseases and even cancer. Methylated DNA presents a new generation of biomarkers which can be used for point-of-care detection. Electrochemical biosensors provide simple, fast, cost-effective, easy-to-use, reliable and efficient detection in contrast with conventional diagnostic methods. These biosensors can be used for early diagnosis of mentioned diseases and increase patient recovery by early clinical interventions. In this study, methylated DNA was detected electrochemically by the developed biosensor. The bare gold electrode was modified by drop-casting reduced graphene oxide with silver nanoparticles which enhance electrochemical signal due to their strong affinity to thiol modified DNA probe via a disulfide bridge. Afterwards, the sensitivity and selectivity of the nanocomposite were examined. The faradic electrochemical impedance spectroscopy was used for determination of the hybridization of the DNA probe with a methylated DNA sequence. The fabricated biosensor shows promising analytical features with a wide detection of the linear range.en
dc.formattextcs
dc.format.extent391-395cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNANOCON 2019 CONFERENCE PROCEEDINGS. 2020, p. 391-395.en
dc.identifier.doi10.37904/nanocon.2019.8610cs
dc.identifier.isbn978-80-87294-95-6cs
dc.identifier.other164255cs
dc.identifier.urihttp://hdl.handle.net/11012/196668
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofNANOCON 2019 CONFERENCE PROCEEDINGScs
dc.relation.urihttps://doi.org/10.37904/nanocon.2019.8610cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectReduced graphene oxideen
dc.subjectsilver nanoparticlesen
dc.subjectnanomaterialsen
dc.subjectmethylated DNAen
dc.subjectelectrochemical biosensoren
dc.titleElectrochemical biosensor based on modified reduced graphene oxide with silver nanoparticles for detection of methylated DNAen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164255en
sync.item.dbtypeVAVen
sync.item.insts2022.03.03 16:55:15en
sync.item.modts2022.03.03 16:15:09en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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