Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

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dc.contributor.authorVaněčková, Terezacs
dc.contributor.authorPompeiano Vaníčková, Luciecs
dc.contributor.authorKuchynka, Michaelacs
dc.contributor.authorPomorski, Adamcs
dc.contributor.authorKrężel, Arturcs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorKanický, Viktorcs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue1cs
dc.coverage.volume198cs
dc.date.issued2019-06-01cs
dc.description.abstractWe report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).en
dc.formattextcs
dc.format.extent224-229cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationTalanta. 2019, vol. 198, issue 1, p. 224-229.en
dc.identifier.doi10.1016/j.talanta.2019.01.089cs
dc.identifier.issn0039-9140cs
dc.identifier.orcid0000-0002-1686-4423cs
dc.identifier.orcid0000-0002-6771-1304cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other156653cs
dc.identifier.researcheridABE-5364-2021cs
dc.identifier.researcheridE-5583-2016cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus53464444600cs
dc.identifier.urihttp://hdl.handle.net/11012/195645
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofTalantacs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0039914019301109cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0039-9140/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectMetallothioneinen
dc.subjectMolecularly imprinted polymersen
dc.subjectPolydopamineen
dc.subjectMALDI-TOP-MSen
dc.subjectLA-ICP-MSen
dc.titleMolecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-156653en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:00en
sync.item.modts2025.01.17 18:36:50en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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