Innovative approach for quantitative determination of ingested microplastics by Daphnia magna: use of differential scanning calorimetry and thermogravimetry

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dc.contributor.authorProcházková, Petracs
dc.contributor.authorKalčíková, Gabrielacs
dc.contributor.authorMaršálková, Eliškacs
dc.contributor.authorZlámalová Gargošová, Helenacs
dc.contributor.authorKučerík, Jiřícs
dc.coverage.issueAprilcs
dc.coverage.volume150cs
dc.date.accessioned2025-06-17T10:58:19Z
dc.date.available2025-06-17T10:58:19Z
dc.date.issued2024-04-08cs
dc.description.abstractDue to the serious environmental impacts of plastic pollution, some conventional plastics have been replaced with biodegradable alternatives. However, these biodegradable options can also fragment and form microplastics, which can be ingested by and harm various biota. The standard methods for quantifying ingested microplastics involve digestion of the gut or the whole organism using acidic, alkaline, or oxidative processes. However, these aggressive methods may destroy biodegradable microplastics, leading to erroneous results. Therefore, in this study, we employed thermal analysis methods, specifically differential scanning calorimetry (DSC) and thermogravimetry (TG), to quantify the ingestion of poly-3-hydroxybutyrate (P3HB), a biodegradable microplastic, by the freshwater crustacean, Daphnia magna. During chronic experiments, we analysed organisms exposed to P3HB with sizes smaller than 125 and 63 m, at concentrations ranging from 1.56–25 mg L1. DSC identified an endothermic peak associated with the melting of semicrystalline P3HB, and its enthalpies were utilised to compute the number/mass of P3HB ingested by D. magna. Notably, shifts in melting points suggested that higher concentrations induced particle agglomeration, and these agglomerates could not penetrate deeply into the organism. The TG approach involved subtracting the mass loss between 200 and 400 °C in D. magna specimens exposed to P3HB suspensions from controls without P3HB exposure. Both methods provided comparable data, revealing that, depending on particle size, individual D. magna ingested up to 10% of their body mass. Our findings indicate that both methods effectively detect P3HB (and potentially other plastic fragments), with DSC demonstrating better sensitivity. While the suggested approach did not enable us to calculate the level of determination or quantification, we were able to demonstrate that DSC can detect P3HB in only one specimen of D. magna exposed to the lowest suspension concentration. This indicates that D. magna exposed to the 63 m fraction ingested approximately 3 g of P3HB, whereas those exposed to the 125 m P3HB ingested around 4 g of P3HB. The introduced methods expand the possibilities for detecting ingested microplastics and probably also nanoplastics, in zooplankton and possibly also other species.en
dc.formattextcs
dc.format.extent1303-1310cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Thermal Analysis and Calorimetry. 2024, vol. 150, issue April, p. 1303-1310.en
dc.identifier.doi10.1007/s10973-024-12985-0cs
dc.identifier.issn1588-2926cs
dc.identifier.orcid0000-0001-9139-6175cs
dc.identifier.orcid0000-0002-8254-2975cs
dc.identifier.orcid0000-0001-8309-8012cs
dc.identifier.orcid0000-0001-9083-4866cs
dc.identifier.other190039cs
dc.identifier.scopus49963776400cs
dc.identifier.scopus7801480372cs
dc.identifier.urihttps://hdl.handle.net/11012/252871
dc.language.isoencs
dc.publisherSpringer Naturecs
dc.relation.ispartofJournal of Thermal Analysis and Calorimetrycs
dc.relation.urihttps://link.springer.com/article/10.1007/s10973-024-12985-0cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1588-2926/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMicroplasticsen
dc.subjectDaphnia magnaen
dc.subjectPoly(3-hydroxybutyrate)en
dc.subjectBiodegradable plasticsen
dc.subjectIngestionen
dc.titleInnovative approach for quantitative determination of ingested microplastics by Daphnia magna: use of differential scanning calorimetry and thermogravimetryen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-190039en
sync.item.dbtypeVAVen
sync.item.insts2025.06.17 12:58:19en
sync.item.modts2025.06.17 12:33:34en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie a technologie ochrany životního prostředícs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
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