Differences in siRNA encapsulation between HsaHFt-RK ferritin and EcaLHFt

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dc.contributor.authorCharousová, Markétacs
dc.contributor.authorMokrý, Michalcs
dc.contributor.authorPekařík, Vladimírcs
dc.date.accessioned2020-01-22T11:54:29Z
dc.date.available2020-01-22T11:54:29Z
dc.date.issued2019-12-31cs
dc.description.abstractNanomedicine in cancer treatment has a great potential. With usage of proper nanocarrier we would be able to eliminate negative side effects and excessively high toxicity of chemotherapeutical drugs to normal cells. Ferritins in general are proteins which could do the trick. Their ability to change structure in low pH could be used for drug delivery. Ferritins are also ubiquitous so there is also very small chance of immune reaction in body. First and the most used and described ferritin is EcaLHFt (ferritin from horse spleen). Its ability to encapsulate various molecules is already known and used in research laboratories. Only molecules with negative charge are troublesome to encapsulate into EcaLHFt. For this reason, we created recombinant human ferritin – HsaHFt-RK, which is designed to encapsulate predominantly negatively charged molecules. We were able to confirm this hypothesis by comparing the encapsulation efficiency of FAM-siRNA inside EcaLHFt and HsaHFt-RK.en
dc.formattextcs
dc.format.extent551-556cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 551-556.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.other161385cs
dc.identifier.urihttp://hdl.handle.net/11012/184129
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0110_Differences-in-siRNA-encapsulation-between-HsaHFt-RK-ferritin-and-EcaLHFt.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectnanomedicineen
dc.subjectferritinen
dc.subjectgene therapyen
dc.subjectsiRNAen
dc.subjectrecombinant proteinen
dc.titleDifferences in siRNA encapsulation between HsaHFt-RK ferritin and EcaLHFten
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161385en
sync.item.dbtypeVAVen
sync.item.insts2021.03.05 12:54:29en
sync.item.modts2021.03.05 12:14:21en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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