Peptide-carbon quantum dots conjugate, derived from human Retinoic Acid Receptor Responder Protein 2, against antibiotic-resistant gram positive and gram negative pathogenic bacteria

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dc.contributor.authorMazumdar, Anindacs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorMichálková, Hanacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorBhowmick, Sukanyacs
dc.contributor.authorMoulick, Amitavacs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.issued2020-02-28cs
dc.description.abstractAntibiotic-resistant bacterial infections have become global issues for public health, which increases the utter need to develop alternatives to antibiotics. Here, the HSER (Homo sapiens retinoic acid receptor) peptide was designed from retinoic acid receptor responder protein 2 of Homo sapiens, and was conjugated with synthesized CQDs (carbon quantum dots) for enhanced antibacterial activity in combination, as individually they are not highly effective. The HSER-CQDs were characterized using spectrophotometer, HPLC coupled with electrospray-ionization quadrupole time-of-flight mass spectrometer (ESI-qTOF) mass spectrometer, zeta potential, zeta size, and FTIR. Thereafter, the antibacterial activity against Vancomycin-Resistant Staphylococcus aureus (VRSA) and Escherichia coli (carbapenem resistant) was studied using growth curve analysis, further supported by microscopic images showing the presence of cell debris and dead bacterial cells. The antibacterial mechanism of HSER-CQDs was observed to be via cell wall disruption and also interaction with gDNA (genomic DNA). Finally, toxicity test against normal human epithelial cells showed no toxicity, confirmed by microscopic analysis. Thus, the HSER-CQDs conjugate, having high stability and low toxicity with prominent antibacterial activity, can be used as a potential antibacterial agent.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNanomaterials. 2020, vol. 10, issue 1, p. 1-19.en
dc.identifier.doi10.3390/nano10020325cs
dc.identifier.issn2079-4991cs
dc.identifier.orcid0000-0002-4985-7115cs
dc.identifier.orcid0000-0002-7844-4336cs
dc.identifier.orcid0000-0003-4122-0694cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.other164052cs
dc.identifier.researcheridJ-2985-2015cs
dc.identifier.researcheridP-3551-2018cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.scopus36070488100cs
dc.identifier.scopus56115133500cs
dc.identifier.scopus55783172600cs
dc.identifier.urihttp://hdl.handle.net/11012/188105
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofNanomaterialscs
dc.relation.urihttps://www.mdpi.com/2079-4991/10/2/325cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2079-4991/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbacterial infectionsen
dc.subjectantibiotic-resistanten
dc.subjectcarbon quantumdotsen
dc.subjectantibacterial activityen
dc.subjecttoxicityen
dc.titlePeptide-carbon quantum dots conjugate, derived from human Retinoic Acid Receptor Responder Protein 2, against antibiotic-resistant gram positive and gram negative pathogenic bacteriaen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-164052en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:56en
sync.item.modts2025.01.17 15:21:11en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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