The effect of coffee supplementation on glutathione and total thiols levels

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dc.contributor.authorBuchtová, Žanetacs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorKudr, Jiřícs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorZítka, Ondřejcs
dc.date.accessioned2018-08-22T14:48:03Z
dc.date.available2018-08-22T14:48:03Z
dc.date.issued2017-12-31cs
dc.description.abstractThe effect of coffee supplementation on glutathione and total thiols levels Antioxidants are very important substances that counteract the formation of free radicals. They are divided into exogenous, such as vitamin C which the body receives with food, and endogenous. One of the most important endogenous antioxidants is glutathione (-glutamyl-L-cysteinyl-glycine), which plays an important role in cellular defense against oxidative damage. Free glutathione is presented within organisms in both reduced (GSH) and oxidized forms (GSSG). Oxidative stress leads to a decrease in GSH level and therefore a GSH/GSSG ratio that can be used as an indicator of oxidative stress and an indicator of various diseases. The aim of presented study was to develop a sampling method for capillary blood testing, where we have found that this amount (15 µl) is sufficient for our testing. Another aim was to supplement group of volunteers with coffee and to determine GSH levels and levels of total thiols after 0, 48 h and 96 h of supplementation in capillary blood. HPLC with electrochemical detection was used for GSH determination and Ellman's method for determination of total thiols. We could see the GSH slight increase as well as the levels of total thiols.en
dc.formattextcs
dc.format.extent843-848cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2017. 2017, p. 843-848.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.other148720cs
dc.identifier.urihttp://hdl.handle.net/11012/84169
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0093_The-effect-of-coffee-supplementation-on-glutathione-and-total-thiols-levels.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectAntioxidanten
dc.subjectcoffeeen
dc.subjectglutathioneen
dc.subjectHPLC-EDen
dc.subjecttotal thiolsen
dc.titleThe effect of coffee supplementation on glutathione and total thiols levelsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-148720en
sync.item.dbtypeVAVen
sync.item.insts2020.03.30 16:58:35en
sync.item.modts2020.03.30 15:50:29en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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