Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation
dc.contributor.author | Šafaříková, Eva | cs |
dc.contributor.author | Ehlich, Jiří | cs |
dc.contributor.author | Stříteský, Stanislav | cs |
dc.contributor.author | Vala, Martin | cs |
dc.contributor.author | Weiter, Martin | cs |
dc.contributor.author | Pacherník, Jiří | cs |
dc.contributor.author | Kubala, Lukáš | cs |
dc.contributor.author | Víteček, Jan | cs |
dc.coverage.issue | 3 | cs |
dc.coverage.volume | 23 | cs |
dc.date.accessioned | 2023-01-18T15:55:11Z | |
dc.date.available | 2023-01-18T15:55:11Z | |
dc.date.issued | 2022-01-20 | cs |
dc.description.abstract | Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine. | en |
dc.format | text | cs |
dc.format.extent | 1-16 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | International Journal of Molecular Sciences. 2022, vol. 23, issue 3, p. 1-16. | en |
dc.identifier.doi | 10.3390/ijms23031107 | cs |
dc.identifier.issn | 1422-0067 | cs |
dc.identifier.other | 177314 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/204601 | |
dc.language.iso | en | cs |
dc.publisher | MDPI | cs |
dc.relation.ispartof | International Journal of Molecular Sciences | cs |
dc.relation.uri | https://www.mdpi.com/1422-0067/23/3/1107 | cs |
dc.rights | Creative Commons Attribution 4.0 International | cs |
dc.rights.access | openAccess | cs |
dc.rights.sherpa | http://www.sherpa.ac.uk/romeo/issn/1422-0067/ | cs |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | cs |
dc.subject | conductive polymer | en |
dc.subject | PEDOT | en |
dc.subject | PSS | en |
dc.subject | screen print | en |
dc.subject | embryonic stem cells | en |
dc.subject | electrostimulation | en |
dc.title | Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation | en |
dc.type.driver | article | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-177314 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2023.01.18 16:55:10 | en |
sync.item.modts | 2023.01.18 16:14:32 | en |
thesis.grantor | Vysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemie | cs |
thesis.grantor | Vysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumu | cs |
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