Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

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dc.contributor.authorPohanka, Miroslavcs
dc.contributor.authorSochor, Jiřícs
dc.contributor.authorRuttkay-Nedecký, Branislavcs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorHubálek, Jaromírcs
dc.contributor.authorStiborová, Mariecs
dc.contributor.authorEckschlager, Tomášcs
dc.contributor.authorKizek, Renécs
dc.coverage.issue3cs
dc.coverage.volume10cs
dc.date.issued2012-05-17cs
dc.description.abstractIn the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes.en
dc.description.abstractIn the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes.en
dc.formattextcs
dc.format.extent155-167cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Applied Biomedicine. 2012, vol. 10, issue 3, p. 155-167.en
dc.identifier.doi10.2478/v10136-012-0006-ycs
dc.identifier.issn1214-021Xcs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0002-7496-2558cs
dc.identifier.other92998cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridD-7753-2012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus57207501711cs
dc.identifier.urihttp://hdl.handle.net/11012/200907
dc.language.isoencs
dc.publisherUniversity of South Bohemiacs
dc.relation.ispartofJournal of Applied Biomedicinecs
dc.relation.urihttps://jab.zsf.jcu.cz/artkey/jab-201203-0004_automated-assay-of-the-potency-of-natural-antioxidants-using-pipetting-robot-and-spectrophotometry.phpcs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1214-021X/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectDPPHen
dc.subjectFRAPen
dc.subjectABTSen
dc.subjectDMPDen
dc.subjectfree radicalsen
dc.subjectantioxidanten
dc.subjectDPPH
dc.subjectFRAP
dc.subjectABTS
dc.subjectDMPD
dc.subjectfree radicals
dc.subjectantioxidant
dc.titleAutomated assay of the potency of natural antioxidants using pipetting robot and spectrophotometryen
dc.title.alternativeAutomated assay of the potency of natural antioxidants using pipetting robot and spectrophotometryen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-92998en
sync.item.dbtypeVAVen
sync.item.insts2025.10.14 15:16:47en
sync.item.modts2025.10.14 09:39:51en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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