Ch(O)P-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomography

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dc.contributor.authorParobková, Viktóriacs
dc.contributor.authorKompaníková, Petracs
dc.contributor.authorLázňovský, Jakubcs
dc.contributor.authorKavková, Michaelacs
dc.contributor.authorHampl, Marekcs
dc.contributor.authorBuchtová, Marcelacs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorBryja, Vítězslavcs
dc.coverage.issue1cs
dc.coverage.volume21cs
dc.date.issued2024-01-24cs
dc.description.abstractThe Hindbrain Choroid Plexus is a complex, cerebrospinal fluid-secreting tissue that projects into the 4th vertebrate brain ventricle. Despite its irreplaceability in the development and homeostasis of the entire central nervous system, the research of Hindbrain Choroid Plexus and other Choroid Plexuses has been neglected by neuroscientists for decades. One of the obstacles is the lack of tools that describe the complex shape of the Hindbrain Choroid Plexus in the context of brain ventricles. Here we introduce an effective tool, termed Ch(O)P-CT, for the noninvasive, X-ray micro-computed tomography-based, three-dimensional visualization and subsequent quantitative spatial morphological analysis of developing mouse Hindbrain Choroid Plexus. Ch(O)P-CT can reliably quantify Hindbrain Choroid Plexus volume, surface area, length, outgrowth angle, the proportion of the ventricular space occupied, asymmetries and general shape alterations in mouse embryos from embryonic day 13.5 onwards. We provide evidence that Ch(O)P-CT is suitable for the unbiased evaluation and detection of the Hindbrain Choroid Plexus alterations within various mutant embryos. We believe, that thanks to its versatility, quantitative nature and the possibility of automation, Ch(O)P-CT will facilitate the analysis of the Hindbrain Choroid Plexus in the mouse models. This will ultimately accelerate the screening of the candidate genes and mechanisms involved in the onset of various Hindbrain Choroid Plexus-related diseases.en
dc.formattextcs
dc.format.extent18cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFluids and Barriers of the CNS. 2024, vol. 21, issue 1, 18 p.en
dc.identifier.doi10.1186/s12987-023-00502-8cs
dc.identifier.issn2045-8118cs
dc.identifier.orcid0009-0004-7996-8328cs
dc.identifier.orcid0000-0002-1323-5269cs
dc.identifier.orcid0000-0001-7435-9292cs
dc.identifier.orcid0000-0003-2948-5198cs
dc.identifier.orcid0000-0002-7397-125Xcs
dc.identifier.other188889cs
dc.identifier.researcheridG-9491-2014cs
dc.identifier.researcheridD-6800-2012cs
dc.identifier.scopus7402184758cs
dc.identifier.urihttp://hdl.handle.net/11012/249332
dc.language.isoencs
dc.publisherBMCcs
dc.relation.ispartofFluids and Barriers of the CNScs
dc.relation.urihttps://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-023-00502-8cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-8118/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectHindbrain choroid plexusen
dc.subjectX-ray micro-computed tomographyen
dc.subject3D visualizationen
dc.subjectMorphometricsen
dc.titleCh(O)P-CT: quantitative morphometrical analysis of the Hindbrain Choroid Plexus by X-ray micro-computed tomographyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-188889en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:48:27en
sync.item.modts2025.01.17 15:21:07en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
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