MALDI-TOF MSI method for determining spatial distribution of infection markers in pulmonary tissues of pigs

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dc.contributor.authorDo, Tomášcs
dc.contributor.authorJarošová, Reacs
dc.contributor.authorIlieva, Ladacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorOndráčková, Petracs
dc.contributor.authorFaldyna, Martincs
dc.contributor.authorSládek, Zbyšekcs
dc.contributor.authorZítka, Ondřejcs
dc.date.issued2019-01-02cs
dc.description.abstractIn recent years the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) is used for molecular mapping of diverse biomarkers such as proteins or peptides in animal/plant tissue sections. It takes full advantage of the benefits of MALDITOF technique which is the ability of rapid measurements of all mass spectra in a wide mass range and detection of analytes molecular weights. Interleukins, the group of mostly proinflammatory cytokines, are the proteins that are produced as immune response on bacterial infection Actinobacillus pleuropneumonia. The aim of this study was to develop a MALDI-TOF MSI method for quantitative visualization of spatial distribution of interleukins and other cell markers of lymphocytes, granulocytes and macrophages in porcine tissues of lymph nodes and lungs. The determination of the spatial distribution of produced proteins will bring further useful knowledge of the pathogenesis of this economically important disease of pigs, which can also contribute to reducing the consumption of antimicrobials.en
dc.formattextcs
dc.format.extent495-500cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2018. 2019, p. 495-500.en
dc.identifier.isbn978-80-7509-597-8cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other156235cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/178381
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2018cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201801-0103_MALDI-TOF-MSI-method-for-determining-spatial-distribution-of-infection-markers-in-pulmonary-tissues-of-pigs.php?back=/magno/mnt/2018/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectmass spectrometry imagingen
dc.subjectcytokinesen
dc.subjectinterleukinsen
dc.subjectinfectionen
dc.subjectpig modelen
dc.titleMALDI-TOF MSI method for determining spatial distribution of infection markers in pulmonary tissues of pigsen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156235en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:48en
sync.item.modts2025.01.17 15:28:09en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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