Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers

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dc.contributor.authorVšianský, Vítcs
dc.contributor.authorSvobodová, Markétacs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorŠtěrbová, Dagmarcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorKostřica, Romcs
dc.contributor.authorSmilek, Pavelcs
dc.contributor.authorPlzák, Jancs
dc.contributor.authorBetka, Jancs
dc.contributor.authorKalfert, Davidcs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorRaudenská, Martinacs
dc.coverage.issue5cs
dc.coverage.volume8cs
dc.date.issued2019-05-31cs
dc.description.abstractDespite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of different metabolites may serve as diagnostic or prognostic markers. Blood amino acids have been identified as promising biomarkers in different cancers before, but little is known about this field in HNSCC. Blood amino acid profiles of 140 HNSCC patients were examined using high-performance liquid chromatography. Cox proportional hazards regression model was used to assess the prognostic value of amino acid concentrations in serum. Colony forming assay was used to identify the effect of amino acids that were significant in Cox proportional hazards regression models on colony forming ability of FaDu and Detroit 562 cell lines. In the multivariable Cox regression model for overall survival (OS), palliative treatment was associated with an unfavourable prognosis while high serum levels of methionine have had a positive prognostic impact. In the relapse-free survival (RFS) multivariable model, methionine was similarly identified as a positive prognostic factor, along with tumor localization in the oropharynx. Oral cavity localization and primary radio(chemo)therapy treatment strategy have been linked to poorer RFS. 1mM serine was shown to support the forming of colonies in both tested HNSCC cell lines. Effect of methionine was exactly the opposite.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationCells. 2019, vol. 8, issue 5, p. 1-12.en
dc.identifier.doi10.3390/cells8050428cs
dc.identifier.issn2073-4409cs
dc.identifier.orcid0000-0002-9658-3444cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.orcid0000-0003-1172-7195cs
dc.identifier.other157419cs
dc.identifier.researcheridD-7638-2012cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridE11072012cs
dc.identifier.researcheridD-9920-2012cs
dc.identifier.scopus55769747816cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/180503
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofCellscs
dc.relation.urihttps://www.mdpi.com/2073-4409/8/5/428cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4409/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjecthead and neck canceren
dc.subjectblood biomarkersen
dc.subjectprognosisen
dc.subjectcolony forming assayen
dc.subjectmetabolismen
dc.subjectamino acidsen
dc.titlePrognostic Significance of Serum Free Amino Acids in Head and Neck Cancersen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-157419en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:51en
sync.item.modts2025.01.17 16:38:42en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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