The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging
dc.contributor.author | Komprda, Tomáš | cs |
dc.contributor.author | Sládek, Zbyšek | cs |
dc.contributor.author | Švehlová, Veronika | cs |
dc.contributor.author | Lacková, Zuzana | cs |
dc.contributor.author | Guráň, Roman | cs |
dc.contributor.author | Do, Tomáš | cs |
dc.contributor.author | Wijacki, Jan | cs |
dc.contributor.author | Buchtová, Marcela | cs |
dc.contributor.author | Neuwirthová, Jana | cs |
dc.contributor.author | Gál, Břetislav | cs |
dc.contributor.author | Adam, Vojtěch | cs |
dc.contributor.author | Zítka, Ondřej | cs |
dc.coverage.issue | 4 | cs |
dc.coverage.volume | 88 | cs |
dc.date.accessioned | 2020-08-04T11:03:49Z | |
dc.date.available | 2020-08-04T11:03:49Z | |
dc.date.issued | 2019-12-01 | cs |
dc.description.abstract | The objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model. | en |
dc.format | text | cs |
dc.format.extent | 443-451 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | ACTA VETERINARIA BRNO. 2019, vol. 88, issue 4, p. 443-451. | en |
dc.identifier.doi | 10.2754/avb201988040443 | cs |
dc.identifier.issn | 0001-7213 | cs |
dc.identifier.other | 162414 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/184648 | |
dc.language.iso | en | cs |
dc.publisher | University of Veterinary and Pharmaceutical Sciences in Brno | cs |
dc.relation.ispartof | ACTA VETERINARIA BRNO | cs |
dc.relation.uri | https://actavet.vfu.cz/88/4/0443/ | cs |
dc.rights | Creative Commons Attribution 4.0 International | cs |
dc.rights.access | openAccess | cs |
dc.rights.sherpa | http://www.sherpa.ac.uk/romeo/issn/0001-7213/ | cs |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | cs |
dc.subject | Docosahexaenoic acid | en |
dc.subject | eicosapentaenoic acid | en |
dc.subject | Schizochytrium oil | en |
dc.subject | reepithelialization | en |
dc.subject | MALDI MSI | en |
dc.subject | collagen alpha-1(III) | en |
dc.title | The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging | en |
dc.type.driver | article | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-162414 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2020.08.04 13:03:49 | en |
sync.item.modts | 2020.08.04 12:34:39 | en |
thesis.grantor | Vysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástroje | cs |
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