The effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imaging

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dc.contributor.authorKomprda, Tomášcs
dc.contributor.authorSládek, Zbyšekcs
dc.contributor.authorŠvehlová, Veronikacs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorDo, Tomášcs
dc.contributor.authorWijacki, Jancs
dc.contributor.authorBuchtová, Marcelacs
dc.contributor.authorNeuwirthová, Janacs
dc.contributor.authorGál, Břetislavcs
dc.contributor.authorAdam, Vojtěchcs
dc.contributor.authorZítka, Ondřejcs
dc.coverage.issue4cs
dc.coverage.volume88cs
dc.date.issued2019-12-01cs
dc.description.abstractThe objective of the present study was to compare the effects of dietary oils containing polyunsaturated fatty acids (PUFA) n-3 and n-6, respectively, on cutaneous wound healing in rats, and to demonstrate the usefulness of the matrix-assisted-laser-desorption-ionization mass spectroscopy-imaging (MALDI MSI) method in this type of experiment. Superiority of PUFA n-3 in this context was the tested hypothesis. Four groups of male Wistar rats by twelve animals each were fed a diet with added 5% of palm oil (P; control), fish oil (F), Schizochytrium microalga oil (Sch) and safflower oil (S), respectively, for eight weeks. Consequently, dorsal full-thickness cutaneous excisions were performed, and selected markers of wound healing were evaluated 18 days post excision. The median of signal intensity corresponding to an amount of collagen alpha-1 (III) fragment, quantified using MALDI MSI, decreased in a sequence P > F > Sch > S (P < 0.001). Using haematoxylin-eosin staining of the histological preparations, semiquantitatively assessed epithelium height tended to decrease in the order of P > S > Sch > F; the wound extent in the sequence of P > Sch > S > F; and the sequence of the progress of neoangiogenesis was assessed as S > P > F approximate to Sch. It was concluded that the tested hypothesis was confirmed only partially: PUFA n-3 showed better results regarding the wound extent, but were inferior in terms of epithelium height and progress of neo-angiogenesis. This was the first time MALDI MSI was successfully employed for evaluating skin wound healing in a rat model.en
dc.formattextcs
dc.format.extent443-451cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationACTA VETERINARIA BRNO. 2019, vol. 88, issue 4, p. 443-451.en
dc.identifier.doi10.2754/avb201988040443cs
dc.identifier.issn0001-7213cs
dc.identifier.orcid0000-0003-4254-5992cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other162414cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/184648
dc.language.isoencs
dc.publisherUniversity of Veterinary and Pharmaceutical Sciences in Brnocs
dc.relation.ispartofACTA VETERINARIA BRNOcs
dc.relation.urihttps://actavet.vfu.cz/88/4/0443/cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0001-7213/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectDocosahexaenoic aciden
dc.subjecteicosapentaenoic aciden
dc.subjectSchizochytrium oilen
dc.subjectreepithelializationen
dc.subjectMALDI MSIen
dc.subjectcollagen alpha-1(III)en
dc.titleThe effect of different fatty acid sources on wound healing in rats assessed by matrix-assisted-laser-desorption-ionization mass-spectroscopy-imagingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-162414en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:56en
sync.item.modts2025.01.17 18:42:26en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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