A non-enzymatic sensor for sensitive determination of H2O2 using biomimetic nanocomposite

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dc.contributor.authorMukherjee, Atripancs
dc.contributor.authorAshrafi, Amirmansoorcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorAdam, Vojtěchcs
dc.date.issued2019-12-31cs
dc.description.abstractHydrogen peroxide (H2O2), as a critical secondary messenger, is important for in vitro and in vivo study. In addition, it is of great significance in monitoring the biological condition under chemical oxidative stress. A non-enzymatic sensor was developed for the detection and monitoring of H2O2 using a nanocomposite (NC). The NC is prepared and modified by using multi-walled carbon nanotubes and tris(2,2’-bipyridyl) copper(II) dichloride complex acts as a mediator. The electrochemical techniques like cyclic voltammetry and chrono-amperometry were used to study the electrochemical behaviour of H2O2 at the developed electrode. The reduction peak of H2O2 shifts to less negative potential with increasing the reducing current at the developed electrode. The calibration curve of the proposed sensor was plotted using chrono-amperometry at 0.1 V as applied potential with stepwise addition of H2O2. The developed sensor has high stability and able to detect H2O2 with a high sensitivity and repeatability. The linear range was found to be 2.4–33.0 µg/ml. Furthermore, the limit of detection and the limit of quantification were 0.7 µg/ml and 2.4 µg/ml, respectively. The reproducibility of the prepared sensor was evaluated by calculating the RSD% = 7.2.en
dc.formattextcs
dc.format.extent585-590cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 585-590.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other161394cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus12040049600cs
dc.identifier.urihttp://hdl.handle.net/11012/184641
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0113_A-non-enzymatic-sensor-for-sensitive-determination-of-H-sub-2-sub-O-sub-2-sub-using-biomimetic-nanocomposite.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectnon-enzymaticen
dc.subjectnanocompositeen
dc.subjectbiomimeticen
dc.subjectnanozymesen
dc.subjectsensorsen
dc.titleA non-enzymatic sensor for sensitive determination of H2O2 using biomimetic nanocompositeen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161394en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:55en
sync.item.modts2025.01.17 20:32:34en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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