Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

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dc.contributor.authorPetrlák, Františekcs
dc.contributor.authorŠmídová, Veronikacs
dc.contributor.authorŠplíchal, Zbyněkcs
dc.contributor.authorMichálek, Petrcs
dc.date.accessioned2020-02-19T15:53:50Z
dc.date.available2020-02-19T15:53:50Z
dc.date.issued2019-12-31cs
dc.description.abstractThis study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.en
dc.formattextcs
dc.format.extent606-611cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 606-611.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.other161395cs
dc.identifier.urihttp://hdl.handle.net/11012/184642
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectbiomarkersen
dc.subjectcisplatinen
dc.subjectmetallothioneinsen
dc.subjectPCRen
dc.subjectprostatic cell linesen
dc.titleOptimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell linesen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161395en
sync.item.dbtypeVAVen
sync.item.insts2021.03.05 12:54:30en
sync.item.modts2021.03.05 12:14:20en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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