Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections

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dc.contributor.authorMazumdar, Anindacs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorSur, Vishma Pratapcs
dc.contributor.authorMilosavljević, Vedrancs
dc.contributor.authorBhowmick, Sukanyacs
dc.contributor.authorMichálková, Hanacs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorVeselý, Radekcs
dc.contributor.authorMoulick, Amitavacs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.issued2020-08-25cs
dc.description.abstractAn inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFrontiers in Microbiology. 2020, vol. 11, issue 1, p. 1-19.en
dc.identifier.doi10.3389/fmicb.2020.01963cs
dc.identifier.issn1664-302Xcs
dc.identifier.orcid0000-0002-4985-7115cs
dc.identifier.orcid0000-0002-7844-4336cs
dc.identifier.orcid0000-0002-1175-7194cs
dc.identifier.orcid0000-0003-4122-0694cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0001-5769-6748cs
dc.identifier.other169138cs
dc.identifier.researcheridJ-2985-2015cs
dc.identifier.researcheridP-3551-2018cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridI-9677-2016cs
dc.identifier.scopus36070488100cs
dc.identifier.scopus56115133500cs
dc.identifier.scopus55783172600cs
dc.identifier.urihttp://hdl.handle.net/11012/196325
dc.language.isoencs
dc.publisherFrontiers Mediacs
dc.relation.ispartofFrontiers in Microbiologycs
dc.relation.urihttps://doi.org/10.3389/fmicb.2020.01963cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1664-302X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectantibacterial peptidesen
dc.subjectantibioticsen
dc.subjectmultidrug resistanceen
dc.subjectbacteriaen
dc.subjectinfectionsen
dc.titleCharacterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infectionsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-169138en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:02en
sync.item.modts2025.01.17 15:25:29en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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