Determination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detector

Simple item page
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorCernei, Natalia Vladimirovnacs
dc.contributor.authorŠtěrbová, Dagmarcs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorRozíková, Veronikacs
dc.contributor.authorKomprda, Tomášcs
dc.contributor.authorZítka, Ondřejcs
dc.date.issued2017-12-31cs
dc.description.abstractThe first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 g/ml whereas the limit of quantification (LOQ) was 13.50 g/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 µl 6M HCl.en
dc.formattextcs
dc.format.extent905-910cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2017. 2017, p. 905-910.en
dc.identifier.isbn978-80-7509-529-9cs
dc.identifier.orcid0000-0003-4254-5992cs
dc.identifier.orcid0000-0002-8259-1566cs
dc.identifier.orcid0000-0002-7844-4336cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other148747cs
dc.identifier.researcheridK-2578-2016cs
dc.identifier.researcheridJ-2985-2015cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus37080103000cs
dc.identifier.scopus36070488100cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/84181
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2017cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201701-0104_Determination-of-hydroxyproline-using-ion-exchange-liquid-chromatography-with-VIS-detector-and-high-performance.php?back=/magno/mnt/2017/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectHydroxyprolineen
dc.subjectHPLC-FLDen
dc.subjectIEC-VISen
dc.subjectmicrowave hydrolysisen
dc.subjectpig skinen
dc.titleDetermination of hydroxyproline using ion-exchange liquid chromatography with vis detector and high performance liquid chromatography with fluorescence detectoren
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-148747en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:27en
sync.item.modts2025.01.18 06:31:58en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
MendelNet_mnt2017010104.pdf
Size:
284.97 KB
Format:
Adobe Portable Document Format
Description:
MendelNet_mnt2017010104.pdf
Download
Collections
Chytré nanonástroje