Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

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dc.contributor.authorNěmčáková, Ivanacs
dc.contributor.authorBlahová, Luciecs
dc.contributor.authorRyšánek, Petrcs
dc.contributor.authorBlanquer, Andreucs
dc.contributor.authorBačáková, Luciecs
dc.contributor.authorZajíčková, Lenkacs
dc.coverage.issue24cs
dc.coverage.volume21cs
dc.date.accessioned2021-03-22T11:54:13Z
dc.date.available2021-03-22T11:54:13Z
dc.date.issued2020-12-01cs
dc.description.abstractAmine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.en
dc.formattextcs
dc.format.extent1-24cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2020, vol. 21, issue 24, p. 1-24.en
dc.identifier.doi10.3390/ijms21249467cs
dc.identifier.issn1422-0067cs
dc.identifier.other169628cs
dc.identifier.urihttp://hdl.handle.net/11012/196474
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttps://www.mdpi.com/1422-0067/21/24/9467cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectamine plasma polymeren
dc.subjectbioactive coatingen
dc.subjectpolycaprolactone nanofibersen
dc.subjectsubstrate morphologyen
dc.subjectcell adhesionen
dc.subjectcell proliferationen
dc.titleBehaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologiesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-169628en
sync.item.dbtypeVAVen
sync.item.insts2021.09.11 16:56:42en
sync.item.modts2021.09.11 16:14:53en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé nízkodimenzionální nanomateriálycs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav teoretické a experimentální elektrotechnikycs
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Ústav teoretické a experimentální elektrotechniky
Pokročilé nízkodimenzionální nanomateriály