Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
dc.contributor.author | Němčáková, Ivana | cs |
dc.contributor.author | Blahová, Lucie | cs |
dc.contributor.author | Ryšánek, Petr | cs |
dc.contributor.author | Blanquer, Andreu | cs |
dc.contributor.author | Bačáková, Lucie | cs |
dc.contributor.author | Zajíčková, Lenka | cs |
dc.coverage.issue | 24 | cs |
dc.coverage.volume | 21 | cs |
dc.date.accessioned | 2021-03-22T11:54:13Z | |
dc.date.available | 2021-03-22T11:54:13Z | |
dc.date.issued | 2020-12-01 | cs |
dc.description.abstract | Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market. | en |
dc.format | text | cs |
dc.format.extent | 1-24 | cs |
dc.format.mimetype | application/pdf | cs |
dc.identifier.citation | International Journal of Molecular Sciences. 2020, vol. 21, issue 24, p. 1-24. | en |
dc.identifier.doi | 10.3390/ijms21249467 | cs |
dc.identifier.issn | 1422-0067 | cs |
dc.identifier.other | 169628 | cs |
dc.identifier.uri | http://hdl.handle.net/11012/196474 | |
dc.language.iso | en | cs |
dc.publisher | MDPI | cs |
dc.relation.ispartof | International Journal of Molecular Sciences | cs |
dc.relation.uri | https://www.mdpi.com/1422-0067/21/24/9467 | cs |
dc.rights | Creative Commons Attribution 4.0 International | cs |
dc.rights.access | openAccess | cs |
dc.rights.sherpa | http://www.sherpa.ac.uk/romeo/issn/1422-0067/ | cs |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | cs |
dc.subject | amine plasma polymer | en |
dc.subject | bioactive coating | en |
dc.subject | polycaprolactone nanofibers | en |
dc.subject | substrate morphology | en |
dc.subject | cell adhesion | en |
dc.subject | cell proliferation | en |
dc.title | Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies | en |
dc.type.driver | article | en |
dc.type.status | Peer-reviewed | en |
dc.type.version | publishedVersion | en |
sync.item.dbid | VAV-169628 | en |
sync.item.dbtype | VAV | en |
sync.item.insts | 2021.09.11 16:56:42 | en |
sync.item.modts | 2021.09.11 16:14:53 | en |
thesis.grantor | Vysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé nízkodimenzionální nanomateriály | cs |
thesis.grantor | Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav teoretické a experimentální elektrotechniky | cs |
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