Genomic and Phenotypic Comparison of Polyhydroxyalkanoates Producing Strains of genus Caldimonas/Schlegelella

Musilová, Jana; Kouřilová, Xenie; Heřmánková, Kristýna; Bezdíček, Matěj; Ieremenko, Anastasiia; Dvořák, Pavel; Obruča, Stanislav; Sedlář, Karel

Genomic and Phenotypic Comparison of Polyhydroxyalkanoates Producing Strains of genus Caldimonas/Schlegelella

Files

1s2.0S2001037023004075main.pdf(3.52 MB)

Date

2023-10-30

Authors

Musilová, Jana

Kouřilová, Xenie

Heřmánková, Kristýna

Bezdíček, Matěj

Ieremenko, Anastasiia

Dvořák, Pavel

Obruča, Stanislav

Sedlář, Karel

ORCID

Publisher

Elsevier

Altmetrics

Abstract

Polyhydroxyalkanoates (PHAs) have emerged as an environmentally friendly alternative to conventional polyesters. In this study, we present a comprehensive analysis of the genomic and phenotypic characteristics of three non-model thermophilic bacteria known for their ability to produce PHAs: Schlegelella aquatica LMG 23380T, Caldimonas thermodepolymerans DSM 15264, and C. thermodepolymerans LMG 21645 and the results were compared with the type strain C. thermodepolymerans DSM 15344T. We have assembled the first complete genomes of these three bacteria and performed the structural and functional annotation. This analysis has provided valuable insights into the biosynthesis of PHAs and has allowed us to propose a comprehensive scheme of carbohydrate metabolism in the studied bacteria. Through phylogenomic analysis, we have confirmed the synonymity between Caldimonas and Schlegelella genera, and further demonstrated that S. aquatica and S. koreensis, currently classified as orphan species, belong to the Caldimonas genus.

Keywords

Caldimonas, Schlegelella, de novo assembly, PHAs, Next-Generation Industrial Biotechnology, LMG 23380T, DSM 15264, LMG 21645, DSM 15344T

Citation

Computational and Structural Biotechnology Journal. 2023, vol. 21, issue November, p. 5372-5381.
https://www.sciencedirect.com/science/article/pii/S2001037023004075

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/