Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

Simple item page
dc.contributor.authorJarošová, Reacs
dc.contributor.authorSmolíková, Vendulacs
dc.contributor.authorDvořák, Marekcs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorDo, Tomášcs
dc.contributor.authorOndráčková, Petracs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorSládek, Zbyšekcs
dc.date.accessioned2019-06-20T06:10:44Z
dc.date.available2019-06-20T06:10:44Z
dc.date.issued2019-01-02cs
dc.description.abstractIn last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.en
dc.formattextcs
dc.format.extent506-511cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2018. 2019, p. 506-511.en
dc.identifier.isbn978-80-7509-597-8cs
dc.identifier.other156237cs
dc.identifier.urihttp://hdl.handle.net/11012/178383
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2018cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201801-0105_Preparation-of-cryosections-from-frozen-porcine-pulmonary-tissue-for-MALDI-mass-spectrometry-imaging.php?back=/magno/mnt/2018/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectcryosectioningen
dc.subjectlungsen
dc.subjectpigen
dc.subjectpleuropneumoniaen
dc.subjectmass spectrometry imagingen
dc.titlePreparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imagingen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156237en
sync.item.dbtypeVAVen
sync.item.insts2020.04.01 11:01:29en
sync.item.modts2020.04.01 05:59:59en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging.pdf
Size:
1023 KB
Format:
Adobe Portable Document Format
Description:
Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging.pdf
Download
Collections
Chytré nanonástroje