Preparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imaging

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dc.contributor.authorJarošová, Reacs
dc.contributor.authorSmolíková, Vendulacs
dc.contributor.authorDvořák, Marekcs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorDo, Tomášcs
dc.contributor.authorOndráčková, Petracs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorSládek, Zbyšekcs
dc.date.issued2019-01-02cs
dc.description.abstractIn last decades, a matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was used for mapping the spatial distribution of different molecules, mainly peptides, proteins, lipids and metabolites, in different types of tissue. Cryosections are the best suited for getting as much information as possible because the preparation of samples for MALDI MSI does not include deparaffinization and antigen-retrieval steps which are needed in case of conventional formalin-fixed and paraffin-embedded (FFPE) tissue sections. To analyse infection markers like interleukins in the tissue sections of porcine lungs affected by Actinobacillus pleuropneumoniae, it was necessary to obtain very well prepared cryosections for MALDI MSI. Therefore, this work was focused on optimization of preparation of frozen porcine lungs and its cryosections with conserved morphological structure.en
dc.formattextcs
dc.format.extent506-511cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2018. 2019, p. 506-511.en
dc.identifier.isbn978-80-7509-597-8cs
dc.identifier.orcid0000-0001-5404-9553cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other156237cs
dc.identifier.researcheridH-8903-2018cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/178383
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2018cs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201801-0105_Preparation-of-cryosections-from-frozen-porcine-pulmonary-tissue-for-MALDI-mass-spectrometry-imaging.php?back=/magno/mnt/2018/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectcryosectioningen
dc.subjectlungsen
dc.subjectpigen
dc.subjectpleuropneumoniaen
dc.subjectmass spectrometry imagingen
dc.titlePreparation of cryosections from frozen porcine pulmonary tissue for MALDI mass spectrometry imagingen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-156237en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:49en
sync.item.modts2025.01.17 16:50:55en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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