A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

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dc.contributor.authorVocetková, Karolínacs
dc.contributor.authorSovková, Věracs
dc.contributor.authorBuzgo, Matějcs
dc.contributor.authorLukášová, Věracs
dc.contributor.authorDivín, Radekcs
dc.contributor.authorRampichová, Michaelacs
dc.contributor.authorBlažek, Pavelcs
dc.contributor.authorZikmund, Tomášcs
dc.contributor.authorKaiser, Jozefcs
dc.contributor.authorKarpíšek, Zdeněkcs
dc.contributor.authorAmler, Evžencs
dc.contributor.authorFilova, Evacs
dc.coverage.issue9cs
dc.coverage.volume10cs
dc.date.issued2020-09-10cs
dc.description.abstractVitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly--caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.en
dc.formattextcs
dc.format.extent1-19cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationNanomaterials. 2020, vol. 10, issue 9, p. 1-19.en
dc.identifier.doi10.3390/nano10091801cs
dc.identifier.issn2079-4991cs
dc.identifier.orcid0000-0002-4029-1389cs
dc.identifier.orcid0000-0003-2948-5198cs
dc.identifier.orcid0000-0002-7397-125Xcs
dc.identifier.orcid0000-0003-1187-2819cs
dc.identifier.other165434cs
dc.identifier.researcheridG-9491-2014cs
dc.identifier.researcheridD-6800-2012cs
dc.identifier.scopus7402184758cs
dc.identifier.urihttp://hdl.handle.net/11012/195709
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofNanomaterialscs
dc.relation.urihttps://www.mdpi.com/2079-4991/10/9/1801cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2079-4991/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectvitiligoen
dc.subjectmelanocyteen
dc.subjectplateletsen
dc.subjectelectrospinningen
dc.subjectcentrifugal spinningen
dc.titleA Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatmenten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-165434en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:48:51en
sync.item.modts2025.01.17 16:48:20en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav matematikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé instrumentace a metody pro charakterizace materiálůcs
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Ústav matematiky
Pokročilé instrumentace a metody pro charakterizace materiálů