Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca<SUP>2+</SUP> Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

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dc.contributor.authorRienmüller, Theresacs
dc.contributor.authorShrestha, Nirojcs
dc.contributor.authorPolz, Mathiascs
dc.contributor.authorStoppacher, Saracs
dc.contributor.authorZiesel, Danielcs
dc.contributor.authorMigliaccio, Ludovicocs
dc.contributor.authorPelzmann, Brigittecs
dc.contributor.authorLang, Petracs
dc.contributor.authorZorn-Pauly, Klauscs
dc.contributor.authorLangthaler, Sonjacs
dc.contributor.authorOpančar, Aleksandarcs
dc.contributor.authorBaumgartner, Christiancs
dc.contributor.authorÜçal, Muammercs
dc.contributor.authorSchindl, Rainercs
dc.contributor.authorDerek, Vedrancs
dc.contributor.authorScheruebel, Susannecs
dc.coverage.issue6cs
dc.coverage.volume71cs
dc.date.accessioned2025-06-16T13:55:56Z
dc.date.available2025-06-16T13:55:56Z
dc.date.issued2024-06-03cs
dc.description.abstractObjective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels (I-Ca,I-L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I-Ca,I-L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I-Ca,I-L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca2+ channel blocker, nifedipine. Light-triggered I-Ca,I-L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I-Ca,I-L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I-Ca,I-L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.en
dc.formattextcs
dc.format.extent1980-1992cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationIEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING. 2024, vol. 71, issue 6, p. 1980-1992.en
dc.identifier.doi10.1109/TBME.2024.3358240cs
dc.identifier.issn0018-9294cs
dc.identifier.orcid0000-0003-3471-1110cs
dc.identifier.other197349cs
dc.identifier.urihttps://hdl.handle.net/11012/252547
dc.language.isoencs
dc.publisherIEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INCcs
dc.relation.ispartofIEEE TRANSACTIONS ON BIOMEDICAL ENGINEERINGcs
dc.relation.urihttps://ieeexplore.ieee.org/document/10473191cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0018-9294/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectBiomedical modeling and simulationen
dc.subjectcalciumen
dc.subjectcardiac physiologyen
dc.subjectelectrophysiologyen
dc.subjectoptoelectronic devicesen
dc.subjectpatch-clampen
dc.subjectvoltage-gated ion channelsen
dc.titleShedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca<SUP>2+</SUP> Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devicesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-197349en
sync.item.dbtypeVAVen
sync.item.insts2025.06.16 15:55:55en
sync.item.modts2025.06.16 15:33:00en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
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