The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus

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dc.contributor.authorKouřilová, Xeniecs
dc.contributor.authorSchwarzerová, Janacs
dc.contributor.authorPernicová, Ivacs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorKrzyžánek, Vladislavcs
dc.contributor.authorNebesářová, Janacs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue5cs
dc.coverage.volume9cs
dc.date.accessioned2021-09-27T07:54:02Z
dc.date.available2021-09-27T07:54:02Z
dc.date.issued2021-05-01cs
dc.description.abstractActinobacteria belonging to the genus Rubrobacter are known for their multi-extremophilic growth conditions-they are highly radiation-resistant, halotolerant, thermotolerant or even thermophilic. This work demonstrates that the members of the genus are capable of accumulating polyhydroxyalkanoates (PHA) since PHA-related genes are widely distributed among Rubrobacter spp. whose complete genome sequences are available in public databases. Interestingly, all Rubrobacter strains possess both class I and class III synthases (PhaC). We have experimentally investigated the PHA accumulation in two thermophilic species, R. xylanophilus and R. spartanus. The PHA content in both strains reached up to 50% of the cell dry mass, both bacteria were able to accumulate PHA consisting of 3-hydroxybutyrate and 3-hydroxyvalerate monomeric units, none other monomers were incorporated into the polymer chain. The capability of PHA accumulation likely contributes to the multi-extremophilic characteristics since it is known that PHA substantially enhances the stress robustness of bacteria. Hence, PHA can be considered as extremolytes enabling adaptation to extreme conditions. Furthermore, due to the high PHA content in biomass, a wide range of utilizable substrates, Gram-stain positivity, and thermophilic features, the Rubrobacter species, in particular Rubrobacter xylanophilus, could be also interesting candidates for industrial production of PHA within the concept of Next-Generation Industrial Biotechnology.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2021, vol. 9, issue 5, p. 1-13.en
dc.identifier.doi10.3390/microorganisms9050909cs
dc.identifier.issn2076-2607cs
dc.identifier.other172539cs
dc.identifier.urihttp://hdl.handle.net/11012/201665
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/2076-2607/9/5/909cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectRubrobacter xylanophilusen
dc.subjectRubrobacter spartanusen
dc.subjectpolyhydroxyalkanoatesen
dc.subjectstress conditionsen
dc.subjectextremophilesen
dc.titleThe First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanusen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172539en
sync.item.dbtypeVAVen
sync.item.insts2021.09.27 09:54:02en
sync.item.modts2021.09.24 16:12:25en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV - laboratoř biotechnologiícs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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Ústav chemie potravin a biotechnologií
Centrum materiálového výzkumu
CMV - laboratoř biotechnologií
Ústav biomedicínského inženýrství