Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis

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dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorPompeiano Vaníčková, Luciecs
dc.contributor.authorHaddad, Yazan Abdulmajeed Eyadhcs
dc.contributor.authorNejdl, Lukášcs
dc.contributor.authorZítka, Jancs
dc.contributor.authorKočiová, Silviacs
dc.contributor.authorMravec, Filipcs
dc.contributor.authorVaculovič, Tomášcs
dc.contributor.authorMacka, Mirekcs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorAdam, Vojtěchcs
dc.coverage.issue1cs
dc.coverage.volume170cs
dc.date.accessioned2022-08-25T14:54:25Z
dc.date.available2022-08-25T14:54:25Z
dc.date.issued2021-02-15cs
dc.description.abstractHerein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) and capillary electrophoresis with LED-induced detection. The FRET pair consisted of quantum dots (QDs) used as an emission tunable donor (emission wavelength of 450 nm) and a cyanine dye (Cy3), providing optimal optical properties as an acceptor. Nonoxidative dimerization of mammalian metallothionein (MT) was investigated using the donor and acceptor covalently conjugated to MT. The main functions of MTs within an organism include the transport and storage of essential metal ions and detoxification of toxic ions. Upon storage under aerobic conditions, MTs form dimers (as well as higher oligomers), which may play an essential role as mediators in oxidoreduction signaling pathways. Due to metal bridging by Cd2+ ions between molecules of metallothionein, the QDs and Cy3 were close enough, enabling a FRET signal. The FRET efficiency was calculated to be in the range of 11–77%. The formation of MT dimers in the presence of Cd2+ ions was confirmed by MALDI-MS analyses. Finally, the process of oligomerization resulting in FRET was monitored by CE, and oligomerization of MT was confirmed.en
dc.formattextcs
dc.format.extent53-60cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2021, vol. 170, issue 1, p. 53-60.en
dc.identifier.doi10.1016/j.ijbiomac.2020.12.105cs
dc.identifier.issn0141-8130cs
dc.identifier.other167427cs
dc.identifier.urihttp://hdl.handle.net/11012/208291
dc.language.isoencs
dc.publisherElseviercs
dc.relation"European Union (EU)" & "Horizon 2020"en
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULEScs
dc.relation.projectIdinfo:eu-repo/grantAgreement/EC/H2020/759585/EU//ToMeTuM
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0141813020352466cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0141-8130/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectFRETen
dc.subjectQuantum dotsen
dc.subjectOligomerizationen
dc.subjectCapillary electrophoresisen
dc.titleMetallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-167427en
sync.item.dbtypeVAVen
sync.item.insts2022.08.25 16:54:25en
sync.item.modts2022.08.25 16:14:16en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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