Comparison of dietary oils with different polyunsaturated fatty acid n-3 and n-6 content in the rat model of cutaneous wound healing

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dc.contributor.authorKomprda, Tomášcs
dc.contributor.authorSládek, Zbyšekcs
dc.contributor.authorŠevčíková, Zuzanacs
dc.contributor.authorŠvehlová, Veronikacs
dc.contributor.authorWijacki, Jancs
dc.contributor.authorGuráň, Romancs
dc.contributor.authorDo, Tomášcs
dc.contributor.authorLacková, Zuzanacs
dc.contributor.authorPolanská, Hanacs
dc.contributor.authorVrlíková, Luciecs
dc.contributor.authorPopelková, Vendulacs
dc.contributor.authorMichálek, Petrcs
dc.contributor.authorZítka, Ondřejcs
dc.contributor.authorBuchtová, Marcelacs
dc.coverage.issue21cs
dc.coverage.volume21cs
dc.date.issued2020-10-24cs
dc.description.abstractDietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen alpha-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).en
dc.formattextcs
dc.format.extent1-22cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2020, vol. 21, issue 21, p. 1-22.en
dc.identifier.doi10.3390/ijms21217911cs
dc.identifier.issn1422-0067cs
dc.identifier.orcid0000-0002-2912-714Xcs
dc.identifier.orcid0000-0003-4254-5992cs
dc.identifier.orcid0000-0002-0121-0128cs
dc.identifier.orcid0000-0001-7607-5058cs
dc.identifier.other166246cs
dc.identifier.researcheridC-2610-2016cs
dc.identifier.researcheridB-9180-2014cs
dc.identifier.researcheridE11072012cs
dc.identifier.scopus14012648400cs
dc.identifier.urihttp://hdl.handle.net/11012/195730
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttps://www.mdpi.com/1422-0067/21/21/7911cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyunsaturated fatty acidsen
dc.subjecthydroxyprolineen
dc.subjectcollagen Ien
dc.subjectIIIen
dc.subjectMPOen
dc.subjectmacrophagesen
dc.subjectmast cellsen
dc.subjectmatrix-assisted-laser-desorption-ionization-mass-spectrometry-imagingen
dc.titleComparison of dietary oils with different polyunsaturated fatty acid n-3 and n-6 content in the rat model of cutaneous wound healingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-166246en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:01en
sync.item.modts2025.01.17 18:48:07en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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